Long non-coding RNA LINC01116 acts as an oncogene in prostate cancer cells through regulation of miR-744-5p/UBE2L3 axis

作者全名："Yu, Shengjie; Yu, Huihong; Zhang, Yuanfeng; Liu, Chuan; Zhang, Weili; Zhang, Yunyun"

作者地址："[Yu, Shengjie; Zhang, Yuanfeng; Liu, Chuan; Zhang, Weili] Chongqing Med Univ, Affiliated Hosp 2, Dept Urol, 76 Linjiang Rd, Chongqing 400016, Peoples R China; [Yu, Huihong] Chongqing Med Univ, Affiliated Hosp 2, Dept Gastroenterol, Chongqing 400016, Peoples R China; [Zhang, Yunyun] Chongqing Univ, Tumor Radiotherapy Ctr, Canc Hosp, 181 Hanyu Rd, Chongqing 400030, Peoples R China"

通信作者："Zhang, WL (corresponding author), Chongqing Med Univ, Affiliated Hosp 2, Dept Urol, 76 Linjiang Rd, Chongqing 400016, Peoples R China.; Zhang, YY (corresponding author), Chongqing Univ, Tumor Radiotherapy Ctr, Canc Hosp, 181 Hanyu Rd, Chongqing 400030, Peoples R China."

来源：CANCER CELL INTERNATIONAL

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000629888200001

JCR分区：Q1

影响因子：5.8

年份：2021

卷号：21

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：LINC01116; miR-744-5p; UBE2L3; Prostate cancer

摘要："BackgroundLong non-coding RNA (lncRNA) has been confirmed to exert a critical effect on the progression of tumors, including prostate cancer. Previous literature has demonstrated LINC01116 involves in activities of multiple cancers. However, the underlying role of LINC01116 in prostate cancer remains unclear.MethodsqRT-PCR measured the expression of LINC01116 in prostate cancer cells. EdU experiment was used to detect cell proliferation. Transwell assays detected cell migration and invasion. Immunofluorescence staining and western blot assays were utilized to measure EMT progress. The binding relationship between RNAs was confirmed by a series of mechanism assays. In addition, rescue experiments were conducted to verify the relationship among RNAs.ResultsLINC01116 was found to be highly expressed in prostate cancer cells. Functional assays indicated that inhibition of LINC01116 could suppress cell proliferation, migration, invasion and EMT progress. Also, miR-744-5p was proven to bind with LINC01116. Moreover, UBE2L3 was verified as the target gene of miR-744-5p. In rescue assays, we discovered that inhibited miR-744-5p or overexpressed UBE2L3 could offset the suppressive influence of silencing LINC01116 on prostate cancer cells.ConclusionOur study suggested that lncRNA LINC01116 acted as an oncogene in prostate cancer and accelerated prostate cancer cell growth through regulating miR-744-5p/UBE2L3 axis."

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