Transcriptome and Differential Methylation Integration Analysis Identified Important Differential Methylation Annotation Genes and Functional Epigenetic Modules Related to Vitiligo

作者全名："Pu, Yihuan; Chen, Xuenuo; Chen, Yangmei; Zhang, Lingzhao; Chen, Jiayi; Zhang, Yujie; Shao, Xinyi; Chen, Jin"

作者地址："[Pu, Yihuan; Chen, Yangmei; Zhang, Lingzhao; Chen, Jiayi; Zhang, Yujie; Shao, Xinyi; Chen, Jin] Chongqing Med Univ, Dept Dermatol, Affiliated Hosp 1, Chongqing, Peoples R China; [Chen, Xuenuo] Chongqing Med Univ, Dept Gastroenterol, Affiliated Hosp 1, Chongqing, Peoples R China"

通信作者："Chen, J (corresponding author), Chongqing Med Univ, Dept Dermatol, Affiliated Hosp 1, Chongqing, Peoples R China."

来源：FRONTIERS IN IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:000631874400001

JCR分区：Q1

影响因子：7.3

年份：2021

卷号：12

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：vitiligo melanocyte; 850K; MDEGs; PPI; functional epigenetic modules

摘要："Vitiligo is an pigmentation disorder caused by a variety of pathogenic factors; its main pathophysiological conditions include oxidative stress, immune activation, and genetic background. Additionally, DNA methylation is often associated with the pathogenesis of vitiligo; however, the underlying mechanism remains unknown. In the present study, we used the Human Methylation 850K BeadChip platform to detect DNA methylation changes in the vitiligo melanocytes. We then integrated the results with the transcriptome data of vitiligo melanocytes and lesions to analyse the correlation between differentially methylated levels and differentially expressed genes. The results showed that there was a significant negative correlation between methylation levels and differentially expressed genes. Subsequently, we enriched GO and KEGG based on methylated differentially expressed genes (MDEGs) using R package ClusterProfiler, and the results were closely related to the pathogenesis of vitiligo. In addition, we also constructed a PPI network of MDEGs and excavated three important functional epigenetic modules, involving a total of 12 (BCL2L1, CDK1, ECT2, HELLS, HSP90AA1, KIF23, MC1R, MLANA, PBK, PTGS2, SOX10, and TYRP1) genes. These genes affect melanocyte melanogenesis, cellular oxidative stress and other important biological processes. Our comprehensive analysis results support the significant contribution of the status of DNA methylation modification to vitiligo, which will help us to better understand the molecular mechanism of vitiligo and explore new therapeutic strategies."

基金机构：National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81773307]; Chongqing Science and Technology CommissionNatural Science Foundation Project of CQ CSTC [cstc2018jcyjAX0195]

基金资助正文："This work was financially supported by the National Natural Science Foundation of China (No. 81773307) and Chongqing Science and Technology Commission (No. cstc2018jcyjAX0195), which offered the financial support."