YTHDF1 Aggravates the Progression of Cervical Cancer Through m(6)A-Mediated Up-Regulation of RANBP2

作者全名："Wang, Haocheng; Luo, Qingya; Kang, Jianyi; Wei, Qinglv; Yang, Yu; Yang, Dan; Liu, Xiaoyi; Liu, Tao; Yi, Ping"

作者地址："[Wang, Haocheng; Wei, Qinglv; Yang, Yu; Yang, Dan; Liu, Xiaoyi; Liu, Tao; Yi, Ping] Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 3, Chongqing, Peoples R China; [Luo, Qingya] Army Med Univ, Dept Pathol, Affiliated Hosp 1, Chongqing, Peoples R China; [Kang, Jianyi] Army Med Univ, Inst Surg Res, Daping Hosp, Chongqing, Peoples R China"

通信作者："Liu, T; Yi, P (corresponding author), Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 3, Chongqing, Peoples R China."

来源：FRONTIERS IN ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000635930900001

JCR分区：Q2

影响因子：4.7

年份：2021

卷号：11

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：cervical cancer; N6-methyladenosine; YTHDF1; tumorigenicity; RANBP2

摘要："N6-methyladenosine (m(6)A) is the most common post-transcriptional modification of RNA in eukaryotes, which has been demonstrated to play important roles in various cancers. YTHDF1 acts as a crucial m(6)A ""reader"" and regulates the fate of m(6)A modified mRNA. However, its role in cervical cancer remains unknown. In this study, we showed that YTHDF1 was highly expressed in cervical cancer, and was closely associated with the poor prognosis of cervical cancer patients. YTHDF1 knockdown suppressed the growth, migration and invasion, and induced apoptosis of cervical cancer cells. Moreover, YTHDF1 knockdown inhibited tumorigenesis of cervical cancer cells in vivo. Through combined on-line data analysis of RIP-seq, meRIP-seq and Ribo-seq upon YTHDF1 knockdown, RANBP2 was identified as the key target of YTHDF1 in cervical cancer cells. YTHDF1 regulated RANBP2 translation in an m(6)A-dependent manner without effect on its mRNA expression. RANBP2 potentiated the growth, migration and invasion of cervical cancer cells. Our study demonstrated the oncogenic role of YTHDF1 in cervical cancer by regulating RANBP2 expression and YTHDF1 represents a potential target for cervical cancer therapy."

基金机构："Natural Science Foundation of Chongqing, ChinaNatural Science Foundation of Chongqing [cstc2020jcyj-msxmX0344]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81902668]; National Key R&D Program of China [2018YFC1313400]"

基金资助正文："This work was sponsored by the Natural Science Foundation of Chongqing, China (cstc2020jcyj-msxmX0344), the National Natural Science Foundation of China (81902668) and the National Key R&D Program of China (2018YFC1313400)."