A Rapid and Efficient Screening System for Neutralizing Antibodies and Its Application for SARS-CoV-2

作者全名："Han, Xiaojian; Wang, Yingming; Li, Shenglong; Hu, Chao; Li, Tingting; Gu, Chenjian; Wang, Kai; Shen, Meiying; Wang, Jianwei; Hu, Jie; Wu, Ruixin; Mu, Song; Gong, Fang; Chen, Qian; Gao, Fengxia; Huang, Jingjing; Long, Yingyi; Luo, Feiyang; Song, Shuyi; Long, Shunhua; Hao, Yanan; Li, Luo; Wu, Yang; Xu, Wei; Cai, Xia; Gao, Qingzhu; Zhang, Guiji; He, Changlong; Deng, Kun; Du, Li; Nai, Yaru; Wang, Wang; Xie, Youhua; Qu, Di; Huang, Ailong; Tang, Ni; Jin, Aishun"

作者地址："[Han, Xiaojian; Wang, Yingming; Li, Shenglong; Hu, Chao; Li, Tingting; Wang, Jianwei; Wu, Ruixin; Mu, Song; Chen, Qian; Gao, Fengxia; Huang, Jingjing; Long, Yingyi; Luo, Feiyang; Song, Shuyi; Long, Shunhua; Hao, Yanan; Li, Luo; Du, Li; Nai, Yaru; Wang, Wang; Jin, Aishun] Chongqing Med Univ, Dept Immunol, Coll Basic Med, Chongqing, Peoples R China; [Han, Xiaojian; Wang, Yingming; Li, Shenglong; Hu, Chao; Li, Tingting; Wang, Jianwei; Wu, Ruixin; Mu, Song; Chen, Qian; Gao, Fengxia; Huang, Jingjing; Long, Yingyi; Luo, Feiyang; Song, Shuyi; Long, Shunhua; Hao, Yanan; Li, Luo; Du, Li; Nai, Yaru; Wang, Wang; Jin, Aishun] Chongqing Med Univ, Chongqing Key Lab Basic & Translat Res Tumor Immu, Chongqing, Peoples R China; [Gu, Chenjian; Wu, Yang; Xu, Wei; Cai, Xia; Xie, Youhua; Qu, Di] Fudan Univ, Shanghai Med Coll, Sch Basic Med Sci, Dept Med Microbiol & Parasitol,Key Lab Med Mol Vi, Shanghai, Peoples R China; [Wang, Kai; Hu, Jie; Gao, Qingzhu; Zhang, Guiji; He, Changlong; Huang, Ailong; Tang, Ni] Chongqing Med Univ, Key Lab Mol Biol Infect Dis, Minist Educ, Chongqing, Peoples R China; [Shen, Meiying] Harbin Med Univ Canc Hosp, Dept Breast Surg, Harbin, Peoples R China; [Gong, Fang] Chongqing Med Univ, Dept Pediat, Yongchuan Hosp, Chongqing, Peoples R China; [Deng, Kun] Chongqing Med Univ, Dept Lab, Affiliated Hosp 3, Chongqing, Peoples R China"

通信作者："Jin, AS (corresponding author), Chongqing Med Univ, Dept Immunol, Coll Basic Med, Chongqing, Peoples R China.; Jin, AS (corresponding author), Chongqing Med Univ, Chongqing Key Lab Basic & Translat Res Tumor Immu, Chongqing, Peoples R China.; Tang, N (corresponding author), Chongqing Med Univ, Key Lab Mol Biol Infect Dis, Minist Educ, Chongqing, Peoples R China."

来源：FRONTIERS IN IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:000636671300001

JCR分区：Q1

影响因子：7.3

年份：2021

卷号：12

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：SARS-CoV-2; neutralizing antibodies; methodology; spike protein; receptor-binding domain (RBD)

摘要："After the pandemic of COVID-19, neutralizing antibodies (NAbs) against SARS-CoV-2 have been developed for the prophylactic and therapeutic purposes. However, few methodologies are described in detail on how to rapidly and efficiently generate effective NAbs to SARS-CoV-2. Here, we integrated and optimized a strategically screening method for NAbs, which has enabled us to obtain SARS-CoV-2 receptor-binding domain (RBD) specific NAbs within 6 days, followed by additional 9 days for antibody production and function analysis. Using this method, we obtained 198 specific Abs against SARS-CoV-2 RBD from the blood samples of COVID-19 convalescent patients, and 96 of them showed neutralizing activity. At least 20% of these NAbs exhibited advanced neutralizing potency and high affinity, with the top two NAbs showing half-maximal inhibitory concentration (IC50) to block authentic SARS-CoV-2 at 9.88 and 11.13 ng/ml, respectively. Altogether, our study provides an effective methodology with high applicable value for discovering potential preventative and therapeutic NAbs for the emerging infectious diseases."

基金机构：Chongqing Medical University fund [X4457]

基金资助正文：This study was supported by Chongqing Medical University fund (X4457) with the donation from Mr. Yuling Feng.