miR-671-5p Attenuates Neuroinflammation via Suppressing NF-kappa B Expression in an Acute Ischemic Stroke Model

作者全名："Deng, Ling; Guo, Yi; Liu, Jingdong; Wang, Xuan; Chen, Sha; Wang, Qian; Rao, Jianyan; Wang, Yuchun; Zuo, Tianrui; Hu, Qingwen; Zhao, Xiahong; Dong, Zhi"

作者地址："[Deng, Ling; Liu, Jingdong; Wang, Xuan; Chen, Sha; Wang, Qian; Rao, Jianyan; Wang, Yuchun; Zuo, Tianrui; Hu, Qingwen; Zhao, Xiahong; Dong, Zhi] Chongqing Med Univ, Key Lab Biochem & Mol Pharmacol, Coll Pharmacol, Chongqing 400016, Peoples R China; [Deng, Ling] Southwest Med Univ, Lib, Luzhou 646000, Sichuan, Peoples R China; [Guo, Yi] Chongqing Univ, Cent Hosp, Dept Radiol, Chongqing 400014, Peoples R China"

通信作者："Dong, Z (corresponding author), Chongqing Med Univ, Key Lab Biochem & Mol Pharmacol, Coll Pharmacol, Chongqing 400016, Peoples R China."

来源：NEUROCHEMICAL RESEARCH

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000641199700003

JCR分区：Q2

影响因子：4.4

年份：2021

卷号：46

期号：7

开始页：1801

结束页：1813

文献类型：Article

关键词：Ischemic stroke; MiR-671-5p; NF-κ B; Neuroinflammation

摘要："This study was designed to investigate the role of miR-671-5p in in vitro and in vivo models of ischemic stroke (IS). Middle cerebral artery occlusion and reperfusion (MCAO/R) in C57BL/6 mice as well as oxygen-glucose deprivation and reoxygenation (OGD/R) in a mouse hippocampal HT22 neuron line were used as in vivo and in vitro models of IS injury, respectively. miR-671-5p agomir, miR-671-5p antagomir, pcDNA3.1-NF-kappa B, and negative controls were transfected into cells using riboFECT CP reagent. miR-671-5p agomir, pcDNA3.1-NF-kappa B, and negative vectors were administered into MCAO/R mice via intracerebroventricular injection. The results showed that miR-671-5p was significantly downregulated and that miR-671-5p agomir alleviated injury and neuroinflammation induced by ischemic reperfusion. A dual-luciferase reporter assay confirmed that NF-kappa B is a direct target of miR-671-5p. Reverse experiments showed that miR-671-5p agomir reduced neuroinflammation via suppression of NF-kappa B expression in both in vitro and in vivo models of IS. Our data suggest that miR-671-5p may be a viable therapeutic target for diminishing neuroinflammation in patients with IS."

基金机构：Research Innovation of Graduate Students in Chongqing [CYB19165]; General Topics of Basic Research and Frontier Exploration in Chongqing [stc2018jcyjAX0378]

基金资助正文：This study was supported by the Research Innovation of Graduate Students in Chongqing (CYB19165) and General Topics of Basic Research and Frontier Exploration in Chongqing (stc2018jcyjAX0378).We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript. We thank all editors and reviewers for their help and patience.