miR-21a inhibits decidual cell apoptosis by targeting Pdcd4

作者全名："Li, Rong; Wen, Yi-Xian; Geng, Yan-Qing; Zhou, Yong-Jiang; Zhang, Yue; Ding, Yu-Bin; Chen, Xue-Mei; Gao, Ru-Fei; He, Jun-Lin; Wang, Ying-Xiong; Liu, Xue-Qing"

作者地址："[Li, Rong; Wen, Yi-Xian; Geng, Yan-Qing; Zhang, Yue; Ding, Yu-Bin; Chen, Xue-Mei; Gao, Ru-Fei; He, Jun-Lin; Wang, Ying-Xiong; Liu, Xue-Qing] Chongqing Med Univ, Sch Publ Hlth & Management, Lab Reprod Biol, Box 197,1 Yixueyuan Rd, Chongqing 400016, Peoples R China; [Zhou, Yong-Jiang] Hainan Med Coll, Sch Publ Hlth, Maternal Child & Adolescent Hlth, 3 Yixueyuan Rd, Longhua Dist 571199, Hainan, Peoples R China; [Li, Rong] Chongqing Med Univ, Dept Obstet, Affiliated Hosp 1, Chongqing 400016, Peoples R China"

通信作者："Liu, XQ (corresponding author), Chongqing Med Univ, Sch Publ Hlth & Management, Lab Reprod Biol, Box 197,1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：GENES & DISEASES

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000644364000008

JCR分区：Q1

影响因子：6.8

年份：2021

卷号：8

期号：2

开始页：171

结束页：180

文献类型：Article

关键词：Decidual cells; Embryo implantation; hsa-miR-21; mmu-miR-21a; Pdcd4

摘要："Decidualization of endometrial stromal cells (ESCs) accompanied with embryo implantation is a key process in mammalian reproduction. Evidence suggests that maintenance of decidual cells function is essential. As a critical part in post-transcriptional gene regulation, microRNAs (miRNAs/miR) have been confirmed to be involved in decidualization. However, whether microRNAs regulate decidual cells function has not been reported. Aiming to clarify the role and potential mechanism of miRNAs in decidual cells, artificial induced decidualization model in mice was established. There are 94 differentially expressed miRNAs (>two-fold change) between decidualized and non-decidualized tissues, including 60 upregulated and 34 downregulated miRNAs. Of the differentially expressed miRNAs, mmu-miR-21a is up-regulated. RT-qPCR also confirmed the up-regulation of mmu-miR-21a following decidualization in vivo and in vitro, and bioinformatic analysis and luciferase activity assay revealed Pdcd4 to be the target gene of mmu-miR-21a. Inhibition of mmu-miR-21a restrained secretory function of decidual cells induced by mESCs, accompanied with increase of Pdcd4 expression and resulted in the increase of cell apoptosis. In addition, we also determined the expression of hsa-miR-21 and Pdcd4 in human proliferative endometrial tissues and decidua tissues. hsa-miR-21 showed higher expression in human decidua tissues compared with proliferative endo-metrial tissues, while expression of Pdcd4 was contrary to that of hsa-miR-21. Similarly, cell apoptosis increased significantly in human endometrial stromal cell line in response to inhibition of hsa-miR-21. Collectively, we conclude that mmu-miR-21a/hsa-miR-21 may play a key role in regulating the function of decidual cells by inhibiting cell apoptosis through targeting Pdcd4. Copyright (C) 2019, Chongqing Medical University. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)."

基金机构：National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31501207]

基金资助正文：This work was supported by the National Natural Science Foundation of China [grant number 31501207].