Osteogenic and antibacterial dual functions of a novel levofloxacin loaded mesoporous silica microspheres/nano-hydroxyapatite/polyurethane composite scaffold

作者全名："Kuang, Zhiping; Dai, Guangming; Wan, Ruijie; Zhang, Dongli; Zhao, Chen; Chen, Cheng; Li, Jidong; Gu, Hongchen; Huang, Wei"

作者地址："[Kuang, Zhiping; Dai, Guangming; Wan, Ruijie; Zhang, Dongli; Zhao, Chen; Chen, Cheng; Huang, Wei] Chongqing Med Univ, Dept Orthoped Surg, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Kuang, Zhiping; Wan, Ruijie] Chongqing Tradit Chinese Med Hosp, Dept Orthopaed Surg, Chongqing 400011, Peoples R China; [Li, Jidong] Sichuan Univ, Res Ctr Nanobiomat, Analyt & Testing Ctr, Chengdu 610065, Sichuan, Peoples R China; [Gu, Hongchen] Shanghai Jiao Tong Univ, Sch Biomed Engn, Med X Res Inst, State Key Lab Oncogenes & Related Genes, Shanghai 200240, Peoples R China"

通信作者："Huang, W (corresponding author), Chongqing Med Univ, Dept Orthoped Surg, Affiliated Hosp 1, Chongqing 400016, Peoples R China."

来源：GENES & DISEASES

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000644364000010

JCR分区：Q1

影响因子：6.8

年份：2021

卷号：8

期号：2

开始页：193

结束页：202

文献类型：Article

关键词：Antibacterial; Composite scaffold; Levofloxacin; Nanobiomaterial; Osteogenesis

摘要："Lev/MSNs/n-HA/PU has been proved to be a novel scaffold material to treat bone defect caused by chronic osteomyelitis. We have previously identified that this material can effectively treat chronic osteomyelitis caused by Staphylococcus aureus in vivo. However, the potential mechanisms of antibacterial and osteogenic induction properties remain unclear. Thus, for osteogenesis property, immunohistochemistry, PCR, and Western blot were performed to detect the expression of osteogenic markers. Furthermore, flow cytometry and TUNEL were applied to analyze MC3T3-E1 proliferation and apoptosis. For antibacterial property, the material was co-cultivated with bacteria, bacterial colony forming units was counted and the release time of the effective levofloxacin was assayed by agar disc-diffusion test. Moreover, scanning electron microscope was applied to observe adhesion of bacteria. In terms of osteogenic induction, we found BMSCs adherently grew more prominently on Lev/MSNs/n-HA/PU. Lev/MSNs/n-HA/PU also enhanced the expression of osteogenic markers including OCN and COL1 alpha 1, as well as effectively promoted the transition from G1 phase to G2 phase. Furthermore, Lev/MSNs/n-HA/PU could reduce apoptosis of MC3T3-E1. Besides, both Lev/MSNs/n-HA/PU and n-HA/PU materials could inhibit bacterial colonies, while Lev/MSNs/n-HA/PU possessed a stronger antibacterial activities, and lower bacterial adhesion than n-HA/PU. These results illustrated that Lev/MSNs/n-HA/PU composite scaffold possess favorable compatibility in vitro, which induce osteogenic differentiation of MSCs, promote proliferation and differentiation of MC3T3-E1, and inhibit apoptosis. Moreover, clear in vitro antibacterial effect of Lev/MSNs/n-HA/PU was also observed. In summary, this study replenishes the potential of Lev/MSNs/n-HA/PU composite scaffold possess dual functions of anti-infection and enhanced osteogenesis for future clinical application. Copyright (C) 2019, Chongqing Medical University. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)."

基金机构：National High Technology Research and Development Program of China (863 Program)National High Technology Research and Development Program of China [2013AA032203]

基金资助正文："This research was funded by the National High Technology Research and Development Program of China (863 Program, grant number 2013AA032203) ."