In-utero exposure to HT-2 toxin affects meiotic progression and early oogenesis in foetal oocytes by increasing oxidative stress

作者全名："Hong, Yi; Mu, Xinyi; Ji, Xingduo; Chen, Xuemei; Geng, Yanqing; Zhang, Yan; Liu, Qiqi; Li, Fangfang; Wang, Yingxiong; He, Junlin"

作者地址："[Hong, Yi; Ji, Xingduo; Chen, Xuemei; Zhang, Yan; Li, Fangfang; He, Junlin] Chongqing Med Univ, Sch Publ Hlth & Management, Lab Reprod Biol, Chongqing 400016, Peoples R China; [Hong, Yi; Mu, Xinyi; Ji, Xingduo; Chen, Xuemei; Geng, Yanqing; Zhang, Yan; Liu, Qiqi; Li, Fangfang; Wang, Yingxiong; He, Junlin] Chongqing Med Univ, Joint Int Res Lab Reprod & Dev, Chongqing 400016, Peoples R China; [Mu, Xinyi; Geng, Yanqing; Liu, Qiqi; Wang, Yingxiong] Chongqing Med Univ, Coll Basic Med, Chongqing 400016, Peoples R China; [Ji, Xingduo] Chenghua Dist Ctr Dis Control & Prevent, Chengdu 610057, Peoples R China"

通信作者："He, JL (corresponding author), Chongqing Med Univ, Sch Publ Hlth & Management, Lab Reprod Biol, Chongqing 400016, Peoples R China."

来源：ENVIRONMENTAL POLLUTION

ESI学科分类：ENVIRONMENT/ECOLOGY

WOS号：WOS:000647790400007

JCR分区：Q1

影响因子：8.9

年份：2021

卷号：279

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：HT-2; Oocytes; Homologous recombination; Mitochondrial apoptosis

摘要："HT-2 toxin (HT-2), a mycotoxin produced by Fusarium species, is detected in a variety of cereal grain based human food and animal feed. Apart from its well-established immunotoxicity and haematotoxicity, it also causes reproductive disorders. In the present study, we revealed the adverse effects of HT-2 on early oogenesis at the foetal stage. Pregnant mice were orally administered with HT-2 for 3 days at mid-gestation. Oocytes from female foetuses exposed to HT-2 displayed defects in meiotic prophase, including unrepaired DNA damage, elevated recombination levels, and reduced expression of meiotic related genes. Subsequently, increased oxidative stress was observed in the foetal ovaries exposed to HT-2, along with the elevated levels of reactive oxygen species, malondialdehyde, catalase, and superoxide dismutase 1/2, thereby resulting in impaired mitochondrial membrane potential and cell apoptosis. Furthermore, pre-treatment with urolithin A, a natural compound with antioxidant activities, partially reversed the delayed meiotic process by alleviating oxidative stress. Since early oogenesis is essential to determine female fertility in adult life, this study indicated that brief maternal exposure to HT-2 toxin may compromise the fertility of a developing female foetus. (c)& nbsp;2021 Elsevier Ltd. All rights reserved."

基金机构："National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [32070851, 31601200]; Natural Science Foundation of ChongqingNatural Science Foundation of Chongqing [cstc2020jcyj-msxmX0135]; scientific and technological research key program of Chongqing Municipal Education Commission [KJZD-K202000403]; Construction Project of Postgraduates Tutor Team of Chongqing [dstd201809]"

基金资助正文："This work was supported by grants from the National Natural Science Foundation of China (Grant No. 32070851, 31601200), Natural Science Foundation of Chongqing (Grant No. cstc2020jcyj-msxmX0135), scientific and technological research key program of Chongqing Municipal Education Commission (Grant No. KJZD-K202000403), Construction Project of Postgraduates Tutor Team of Chongqing (Grant no. dstd201809)."