Epigallocatechin-3-gallate induces autophagy-related apoptosis associated with LC3B II and Beclin expression of bladder cancer cells

作者全名："Yin, Zhaofa; Li, Juan; Kang, Le; Liu, Xiangyang; Luo, Jianguo; Zhang, Ling; Li, Yuting; Cai, Jiarong"

作者地址："[Yin, Zhaofa; Kang, Le; Luo, Jianguo; Zhang, Ling; Li, Yuting; Cai, Jiarong] Loudi Cent Hosp Hunan Prov, Dept Urol, Loudi, Peoples R China; [Li, Juan] Chongqing Med Univ, Affiliated Hosp 2, Dept Digest, Chongqing, Peoples R China; [Liu, Xiangyang] Loudi Cent Hosp Hunan Prov, Dept Ultrasound, Loudi, Peoples R China"

通信作者："Kang, L (corresponding author), Cent Hosp Loudi City, Dept Urol, 51 Middle Changqing St, Loudi 417000, Hunan, Peoples R China."

来源：JOURNAL OF FOOD BIOCHEMISTRY

ESI学科分类：AGRICULTURAL SCIENCES

WOS号：WOS:000651012800001

JCR分区：Q2

影响因子：4

年份：2021

卷号：45

期号：6

开始页：　

结束页：　

文献类型：Article

关键词：autophagy; apoptosis; bladder cancer; epigallocatechin‐ 3‐ gallate

摘要："The incidence of bladder cancer in traditional green tea-consuming countries was dramatically lower than low green tea-consuming countries. Epigallocatechin-3-gallate (EGCG), an active ingredient extracted from green tea, showed effective inhibition of formation and progression of many tumors. However, whether autophagy involved in this tumor-suppression mechanism of EGCG on bladder cancer was still unclear. In this study, we demonstrated low concentration of EGCG-induced proliferation inhibition and increased apoptosis in bladder cancer cell lines (5,637 and T24 cells) indicated by the increased expression of apoptosis-related protein (caspase9, caspase3 and BAX). In addition, low dose of EGCG also regulated autophagy pathway associated protein (LC3B II and Beclin) expression and this autophagy pathway was blocked by PI3K/AKT inhibitor; moreover, knockdown of ATG5 reversed EGCG-induced apoptosis in 5,637 cells, indicating that EGCG might inhibit the bladder cancer through autophagy pathway. Our findings indicated that EGCG should be considered as a novel therapy for bladder cancer treatment by regulating autophagy pathway. Practical applications Our research proved EGCG from green tea could be used as an effective anti-tumor ingredient by revealing another mechanism that epigallocatechin-3-Gallate inhibited bladder cancer cells via inducing autophagy-related apoptosis. And green tea could be considered as a kind of tumor-preventing beverage."

基金机构：Technology and Planning Project of Science and Technology Bureau [2018 (50)]

基金资助正文："Technology and Planning Project of Science and Technology Bureau, Grant/Award Number: 2018 (50)"