MiR-706 alleviates white matter injury via downregulating PKC alpha/MST1/NF-kappa B pathway after subarachnoid hemorrhage in mice

作者全名："Ru, Xufang; Qu, Jie; Li, Qiang; Zhou, Jiru; Huang, Suna; Li, Wenyan; Zuo, Shilun; Chen, Yujie; Liu, Zhi; Feng, Hua"

作者地址："[Ru, Xufang; Qu, Jie; Li, Qiang; Zhou, Jiru; Huang, Suna; Li, Wenyan; Chen, Yujie; Liu, Zhi; Feng, Hua] Third Mil Med Univ, Southwest Hosp, Army Mil Med Univ, Dept Neurosurg, Chongqing 400038, Peoples R China; [Ru, Xufang; Qu, Jie; Li, Qiang; Zhou, Jiru; Huang, Suna; Li, Wenyan; Chen, Yujie; Liu, Zhi; Feng, Hua] Third Mil Med Univ, Southwest Hosp, Army Mil Med Univ, State Key Lab Trauma Burn & Combined Injury, Chongqing 400038, Peoples R China; [Ru, Xufang; Qu, Jie; Li, Qiang; Zhou, Jiru; Huang, Suna; Li, Wenyan; Chen, Yujie; Liu, Zhi; Feng, Hua] Third Mil Med Univ, Southwest Hosp, Army Mil Med Univ, Chongqing Key Lab Precis Neuromed & Neuroregenara, Chongqing 400038, Peoples R China; [Qu, Jie] Chinese Peoples Liberat Army Gen Hosp, Med Ctr 6, Dept Emergency, Beijing 100048, Peoples R China; [Zhou, Jiru] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurosurg, Chongqing 400016, Peoples R China; [Zuo, Shilun] Third Mil Med Univ, Xinqiao Hosp, Dept Neurol, Army Med Univ, Chongqing 400037, Peoples R China"

通信作者："Chen, YJ; Liu, Z (corresponding author), Third Mil Med Univ, Southwest Hosp, Army Mil Med Univ, Dept Neurosurg, Chongqing 400038, Peoples R China.; Chen, YJ; Liu, Z (corresponding author), Third Mil Med Univ, Southwest Hosp, Army Mil Med Univ, State Key Lab Trauma Burn & Combined Injury, Chongqing 400038, Peoples R China."

来源：EXPERIMENTAL NEUROLOGY

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000654996800001

JCR分区：Q1

影响因子：5.3

年份：2021

卷号：341

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Subarachnoid hemorrhage; White matter injury; MiR-706; Protein kinase C-alpha; Neuroinflammation

摘要："Increasing numbers of patients with spontaneous subarachnoid hemorrhage(SAH) who recover from surgery and intensive care management still live with cognitive impairment after discharge, indicating the importance of white matter injury at the acute stage of SAH. In the present study, standard endovascular perforation was employed to establish an SAH mouse model, and a microRNA (miRNA) chip was used to analyze the changes in gene expression in white matter tissue after SAH. The data indicate that 17 miRNAs were downregulated, including miR-706, miR-669a-5p, miR-669p-5p, miR-7116-5p and miR-195a-3p, while 13 miRNAs were upregulated, including miR-6907-5p, miR-5135, miR-6982-5p, miR-668-5p, miR-8119. Strikingly, miR-706 was significantly downregulated with the highest fold change. Further experiments confirmed that miR-706 could alleviate white matter injury and improve neurological behavior, at least partially by inhibiting the PKC alpha/MST1/NF-kappa B pathway and the release of inflammatory cytokines. These results might provide a deeper understanding of the pathophysiological processes in white matter after SAH, as well as potential therapeutic strategies for the translational research."

基金机构："National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [82030036, 81901216]; Natural Science Foundation of ChongqingNatural Science Foundation of Chongqing [cstc2019jcyj-msxmX0535]; State Key Laboratory of Trauma, Burn and Combined Injury [SKLYQ202002]; Southwest Hospital [SWH2018BJKJ-05]"

基金资助正文："This work was supported by National Natural Science Foundation of China (82030036, 81901216), Natural Science Foundation of Chongqing (cstc2019jcyj-msxmX0535), State Key Laboratory of Trauma, Burn and Combined Injury (SKLYQ202002) and Southwest Hospital (SWH2018BJKJ-05)."