Astragalus polysaccharide attenuates LPS-related inflammatory osteolysis by suppressing osteoclastogenesis by reducing the MAPK signalling pathway

作者全名："Yang, Jianye; Qin, Leilei; Huang, Jiaxing; Li, Yuwan; Xu, Sha; Wang, Hai; Zhu, Sizheng; Wang, Jiawei; Zhu, Bo; Li, Feilong; Huang, Wei; Gong, Xuan; Hu, Ning"

作者地址："[Yang, Jianye; Qin, Leilei; Huang, Jiaxing; Zhu, Sizheng; Wang, Jiawei; Zhu, Bo; Huang, Wei; Hu, Ning] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthopaed, Chongqing, Peoples R China; [Yang, Jianye; Wang, Hai] Fuling Cent Hosp Chongqing, Dept Orthopaed, Chongqing, Peoples R China; [Li, Yuwan] Peking Univ Third Hosp, Beijing Key Lab Sports Injuries, Inst Sports Med, Beijing, Peoples R China; [Xu, Sha] Banan Second Peoples Hosp Chongqing, Dept Rehabil Med, Chongqing, Peoples R China; [Li, Feilong] Chongqing Dazu Peoples Hosp, Dept Orthopaed, Chongqing, Peoples R China; [Gong, Xuan] Chongqing Gen Hosp, Outpatient Dept, Chongqing 400013, Peoples R China"

通信作者："Hu, N (corresponding author), Chongqing Med Univ, Affiliated Hosp 1, Dept Orthopaed, Chongqing, Peoples R China.; Gong, X (corresponding author), Chongqing Gen Hosp, Outpatient Dept, Chongqing 400013, Peoples R China."

来源：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000657156900001

JCR分区：Q2

影响因子：5.3

年份：2021

卷号：25

期号：14

开始页：6800

结束页：6814

文献类型：Article

关键词：Astragalus polysaccharide; inflammatory osteolysis; lipopolysaccharide; osteoclastogenesis

摘要："Bacterial products can stimulate inflammatory reaction and activate immune cells to enhance the production of inflammatory cytokines, and finally promote osteoclasts recruitment and activity, leading to bone destruction. Unfortunately, effective preventive and treatment measures for inflammatory osteolysis are limited and usually confuse the orthopedist. Astragalus polysaccharide (APS), the main extractive of Astragali Radix, has been widely used for treating inflammatory diseases. In the current study, in vitro and in vivo experimental results demonstrated that APS notably inhibited osteoclast formation and differentiation dose-dependently. Moreover, we found that APS down-regulated RANKL-related osteoclastogenesis and levels of osteoclast marker genes, such as NFATC1, TRAP, c-FOS and cathepsin K. Further underlying mechanism investigation revealed that APS attenuated activity of MAPK signalling pathways (eg ERK, JNK and p38) and ROS production induced by RANKL. Additionally, APS was also found to suppress LPS-related inflammatory osteolysis by decreasing inflammatory factors' production in vivo. Overall, our findings demonstrate that APS effectively down-regulates inflammatory osteolysis due to osteoclast differentiation and has the potential to become an effective treatment of the disorders associated with osteoclast."

基金机构：National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81672167]

基金资助正文："National Natural Science Foundation of China, Grant/Award Number: 81672167"