Simiao Wan attenuates monosodium urate crystal-induced arthritis in rats through contributing to macrophage M2 polarization

作者全名："Yang, Juan; Chen, Gang; Guo, Ting-Wang; Qin, Wen-Yi; Jia, Ping"

作者地址："[Yang, Juan; Qin, Wen-Yi; Jia, Ping] Chongqing Med Univ, Dept Combinat Chinese & Western Med, Affiliated Hosp 1, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China; [Chen, Gang; Guo, Ting-Wang] Chongqing Technol & Business Univ, Coll Environm & Resources, Key Lab Nat Med Res, Chongqing Educ Commiss, Chongqing 400067, Peoples R China"

通信作者："Jia, P (corresponding author), Chongqing Med Univ, Dept Combinat Chinese & Western Med, Affiliated Hosp 1, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：JOURNAL OF ETHNOPHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000657652500004

JCR分区：Q1

影响因子：5.4

年份：2021

卷号：275

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Simiao Wan; Gouty arthritis; MSU-Induced arthritis; Macrophage polarization

摘要："Ethnopharmacological relevance: Simiao Wan (SMW) is a classical traditional Chinese medicine (TCM) prescription to empirically treat gouty arthritis (GA) in TCM clinical practice. However, the potential mechanisms of SMW on GA are not fully evaluated. Aim of study: The aim of this study is to investigate the role of macrophage polarization in the anti-GA activity of SMW. Materials and methods: Rats were intragastricly treated with SMW for consecutive 7 days. On day 6, monosodium urate (MSU) crystal-induced arthritis (MIA) in the ankle joint was prepared. Paw volume, gait score and histological score were measured. Levels of interleukin (IL)-1 beta and IL-10 in serum were detected by enzyme-linked immunosorbent assay. Expressions of inducible nitric oxide synthase (iNOS), arginase (Arg)-1, phosphorylated (p)-p65, inhibitor of nuclear factor (NF)-xB (IxB)alpha, p-signal transducer and transcription activator (STAT)3 and pJanus kinase (JAK)2 in synovial tissues were determined by Western blot. Results: The elevated paw volume, gait score and histological score in MIA rats were significantly decreased by SMW treatment. Meanwhile, SMW significantly decreased the IL-1 beta level and increased the IL-10 level in serum of MIA rats. Furthermore, SMW reduced the expressions of iNOS, p-p65 and enhanced the expressions of Arg-1, IxB alpha, p-STAT3 and p-JAK2 in synovial tissues of MIA rats. Conclusions: The results suggest that SMW attenuates the inflammation in MIA rats through promoting macrophage M2 polarization."

基金机构：Key Program of Health Commission of Chongqing City [ZY201801008]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81503420]; Key Laboratory of Natural Medicine Research of Chongqing Education Commission [KFJJ2018088]

基金资助正文："This work was supported by the Key Program of Health Commission of Chongqing City (ZY201801008), National Natural Science Foundation of China (81503420) and Key Laboratory of Natural Medicine Research of Chongqing Education Commission (KFJJ2018088)."