Microorganisms as Bio-SPE Materials for Extraction of Pharmaceutical Drugs: Mechanism of Extraction

作者全名："Liu, Yi; Luo, Xinxin; Wang, Min; Xia, Zhining; Huang, Yike"

作者地址："[Liu, Yi; Huang, Yike] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China; [Liu, Yi; Luo, Xinxin; Wang, Min; Xia, Zhining] Chongqing Univ, Sch Pharmaceut Sci, Chongqing 401331, Peoples R China"

通信作者："Huang, YK (corresponding author), Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China.; Xia, ZN (corresponding author), Chongqing Univ, Sch Pharmaceut Sci, Chongqing 401331, Peoples R China."

来源：ANALYTICAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:000659336200016

JCR分区：Q1

影响因子：7.4

年份：2021

卷号：93

期号：21

开始页：7665

结束页：7672

文献类型：Article

关键词：　

摘要："In solid-phase extraction (SPE), the extraction materials depend on the physicochemical interactions to obtain the target analytes from complex systems. However, many matrix interferences existing in real samples influence the extraction efficiency through these common interactions. Therefore, extraction materials based on more special interactions for biological systems need to be developed. In this work, live microorganisms including Escherichia coli and Staphylococcus aureus were considered as the potential biological SPE (bio-SPE) materials with their biological functions in the live state. To study the enrichment and selectivity of the bio-SPE, four antibacterial drugs and two non-antibacterial drugs were employed as the target analytes. The enrichment factor (EF) was used as the evaluation index. The results showed that when using chlorpheniramine (CPM) and ofloxacin (OFLO), the enrichment capacity of E. coli was better than that of S. aureus. When extracting a single analyte, the enrichment ability of E. coli for CPM was significantly higher than other analytes, and the EF was 8.5. In a mixture solution of antibacterial analytes, OFLO could be enriched mostly by E. coli. However, in the mixture solution of antibacterial and non-antibacterial analytes, CPM was enriched more than that of antibacterial analytes. In real rat plasma, bio-SPE using live E. coli could obviously extract CPM, while traditional liquid-liquid extraction could not. The confocal microscopy results showed that the extraction mechanism may not only depend on the surface adsorption of bacteria with analytes but also on the uptake into bacteria. This provides a valuable basis for the development of more biological separation materials based on biological interactions."

基金机构："National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [21974015, 21705012]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (NSFC, Nos. 21974015 and 21705012)."