Drowning and postmortem immersion identification using attenuated total reflection-Fourier transform infrared spectroscopy

作者全名："Xiong, Hongli; Wang, Qi; Zhao, Minzhu; Zheng, Zhe; Zhu, Shisheng; Zhu, Ying; Li, Yongguo; Li, Siqiao; Ding, Shijia; Li, Hongwei; Li, Jianbo"

作者地址："[Xiong, Hongli; Wang, Qi; Zhao, Minzhu; Zheng, Zhe; Zhu, Ying; Li, Yongguo; Li, Siqiao; Ding, Shijia; Li, Jianbo] Chongqing Med Univ, Dept Forens Med, Fac Basic Med Sci, Chongqing 400016, Peoples R China; [Zhu, Shisheng] Chongqing Med & Pharmaceut Coll, Fac Med Technol, Chongqing, Peoples R China; [Li, Hongwei] Chongqing Publ Bur, Tech Dept Interpol Corps, Chongqing, Peoples R China"

通信作者："Li, JB (corresponding author), Chongqing Med Univ, Dept Forens Med, Fac Basic Med Sci, Chongqing 400016, Peoples R China."

来源：MICROCHEMICAL JOURNAL

ESI学科分类：CHEMISTRY

WOS号：WOS:000662631300004

JCR分区：Q1

影响因子：4.8

年份：2021

卷号：167

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Drowning; Postmortem immersion; ATR-FTIR; PCA; PLS-DA

摘要："It remains a major challenge for forensic practice to accurately distinguish whether a corpse in the water died from drowning or postmortem immersion. When the corpse is severely decomposed, the problem becomes more complicated. In this study, we utilized attenuated total reflection-Fourier Transform Infrared Spectroscopy (ATRFTIR) and chemometrics to distinguish whether a corpse in the water died from drowning or postmortem immersion. The results of Principal Component Analysis (PCA) showed that there was a certain degree of difference between the freshwater group and the saltwater group due to the different concentrations of drowning medium, and the degradation of lung tissue had no significant effect on the differentiation between drowning and postmortem immersion. Amide I and amide II contribute to the distinction between drowning and postmortem immersion. Subsequently, we constructed partial least squares discriminant analysis (PLS-DA) models for the identification of drowning and posthumous immersion in the freshwater group and the saltwater group, respectively. Both models showed good classification capacity, with the accuracy rates of 94.4% and 100%, respectively. As to results of average second derivative spectroscopy, PCA and PLS-DA all indicate that the difference in protein structure and content is the key to distinguish between drowning and postmortem immersion. This pilot study demonstrates that ATR-FTIR in combination with chemometrics has the potential to be an effective method for distinguishing between drowning and postmortem immersion."

基金机构：Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJQN201902804]; Joint Medical Research of Chongqing Science and Technology Bureau & Chongqing Municipal Health Commission [2019MSXM033]

基金资助正文："This research was supported by the Scientific and Technological Research Program of Chongqing Municipal Education Commission (KJQN201902804) , the Joint Medical Research of Chongqing Science and Technology Bureau & Chongqing Municipal Health Commission (2019MSXM033) ."