Fucoxanthin rescues dexamethasone induced C2C12 myotubes atrophy

作者全名：Liao Zhiyin; Chen Jinliang; Chen Qiunan; Yang Yunfei; Xiao Qian

作者地址："[Liao Zhiyin; Chen Jinliang; Chen Qiunan; Yang Yunfei; Xiao Qian] Chongqing Med Univ, Dept Geriatr, Affiliated Hosp 1, Friendship Rd 1, Chongqing 400016, Peoples R China"

通信作者："Xiao, Q (corresponding author), Chongqing Med Univ, Dept Geriatr, Affiliated Hosp 1, Friendship Rd 1, Chongqing 400016, Peoples R China."

来源：BIOMEDICINE & PHARMACOTHERAPY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000663691300004

JCR分区：Q1

影响因子：7.5

年份：2021

卷号：139

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Dexamethasone; Atrophy; Fucoxanthin; SIRT1

摘要："Muscle atrophy and weakness are the adverse effects of long-term or high dose usage of glucocorticoids. In the present study, we explored the effects of fucoxanthin (10 mu M) on dexamethasone (10 mu M)-induced atrophy in C2C12 myotubes and investigated its underlying mechanisms. The diameter of myotubes was observed under a light microscope, and the expression of myosin heavy chain (MyHC), proteolysis-related, autophagy-related, apoptosis-related, and mitochondria-related proteins was analyzed by western blots or immunoprecipitation. Fucoxanthin alleviates dexamethasone-induced muscle atrophy in C2C12 myotubes, indicated by increased myotubes diameter and expression of MyHC, decreased expression of muscle atrophy F-box (Atrogin-1) and muscle ring finger 1 (MuRF1). Through activating SIRT1, fucoxanthin inhibits forkhead box O (FoxO) transcriptional activity to reduce protein degradation, induces autophagy to enhance degraded protein clearance, promotes mitochondrial function and diminishes apoptosis. In conclusion, we identified fucoxanthin ameliorates dexamethasone induced C2C12 myotubes atrophy through SIRT1 activation."

基金机构："National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81801385, 81701382]"

基金资助正文：This work was financially supported by the National Natural Science Foundation of China (No. 81801385 and No. 81701382) . The authors would like to thank the Research Center of the First Affiliated Hospital of Chongqing Medical University for providing experimental platform.