Absence of Toll-like receptor 7 protects mice against Pseudomonas aeruginosa pneumonia

作者全名："Xu, Haofeng; Huang, Lili; Luo, Qin; Tu, Qianqian; Liu, Jiayu; Yu, Renlin; Huang, Jun; Chen, Te; Yin, Yibing; Cao, Ju"

作者地址："[Xu, Haofeng; Huang, Lili; Yu, Renlin; Huang, Jun; Chen, Te; Cao, Ju] Chongqing Med Univ, Affiliated Hosp 1, Dept Lab Med, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Xu, Haofeng; Tu, Qianqian; Liu, Jiayu; Chen, Te; Yin, Yibing] Chongqing Med Univ, Minist Educ, Key Lab Diagnost Med, Chongqing 400016, Peoples R China; [Luo, Qin] Chongqing Med Univ, Affiliated Hosp 1, Dept Clin Mol, Med Testing Ctr, Chongqing 400016, Peoples R China"

通信作者："Cao, J (corresponding author), Chongqing Med Univ, Affiliated Hosp 1, Dept Lab Med, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：INTERNATIONAL IMMUNOPHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000664073100002

JCR分区：Q1

影响因子：5.6

年份：2021

卷号：96

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Pneumonia; TLR7; Immunity; Pseudomonas aeruginosa; IL-10

摘要："Toll-like receptor 7 (TLR7) is a sensor of microbial ssRNA that participates in the immune response process in many diseases. We herein sought to establish the role of TLR7 in Pseudomonas aeruginosa pneumonia. Pneumonia model was created by intratracheally injecting Pseudomonas aeruginosa and the effects of TLR7 on survival, bacterial burden, lung pathology, cytokine and chemokine production, and pulmonary leukocyte recruitment were measured after Pseudomonas aeruginosa challenge. TLR7 expression was significantly elevated in WT mice after Pseudomonas aeruginosa infection. TLR7-/- mice demonstrated enhanced survival, bacterial clearance, leukocyte infiltration, and macrophages phagocytic activity, and decreased pathology and capillary leakage. Besides, improved survival and bacterial clearance were observed in WT mice treated with TLR7 antagonist IRS661. More importantly, lack of TLR7 suppressed pro-inflammatory cytokine production and induced antiinflammatory cytokine production in mice lungs. Finally, neutralized IL-10 damaged the bacterial clearance ability of TLR7 deficient mice, leading to decreased survival. Collectively, absence of TLR7 provided protective effects during Pseudomonas aeruginosa pneumonia and suggested that TLR7 could act as a novel immune target to treat clinical cases with Pseudomonas aeruginosa pneumonia."

基金机构：National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [82070014]

基金资助正文：This study was supported by research funding from the National Natural Science Foundation of China (No. 82070014) .