Multifunctional electrochemical biosensor with "tetrahedral tripods" assisted multiple tandem hairpins assembly for ultra-sensitive detection of target DNA

作者全名："Huang, Yuqi; Zhao, Shuhui; Zhang, Wenxiu; Duan, Qiuyue; Yan, Qi; Fu, Hu; Zhong, Liang; Yi, Gang"

作者地址："[Huang, Yuqi; Zhao, Shuhui; Zhang, Wenxiu; Duan, Qiuyue; Yan, Qi; Zhong, Liang; Yi, Gang] Chongqing Med Univ, Dept Lab Med, Minist Educ, Key Lab Med Diagnost, Chongqing 400016, Peoples R China; [Fu, Hu] Chengdu First Peoples Hosp, Clin Lab, Chengdu 610000, Peoples R China"

通信作者："Yi, G (corresponding author), Chongqing Med Univ, Dept Lab Med, Minist Educ, Key Lab Med Diagnost, Chongqing 400016, Peoples R China."

来源：RSC ADVANCES

ESI学科分类：CHEMISTRY

WOS号：WOS:000664284900009

JCR分区：Q2

影响因子：3.9

年份：2021

卷号：11

期号：33

开始页：20046

结束页：20056

文献类型：Article

关键词：　

摘要："Nucleic acids are genetic materials in the human body that play important roles in storing, copying, and transmitting genetic information. Abnormal nucleic acid sequences, base mutations, and genetic changes often lead to cancer and other diseases. Meanwhile, methylated DNA is one of the main epigenetic modifications, which is considered to be an excellent biomarker in the early detection, prognosis, and treatment of cancers. Therefore, a multifunctional electrochemical biosensor was constructed with sturdy tetrahedral tripods, which assisted multiple tandem hairpins through base complementary pairing and effective ultra-sensitive detection of targets (DNA, microRNA, and methylated DNA). In the experiments, experimental conditions were optimized, and different DNA concentrations in serum were detected to verify the sensitivity of the biosensor and the feasibility of this protocol. In addition, microRNA and DNA methylation were detected through different designs of tetrahedral tripods (TTs) that capture probes to prove the superiority of this scheme. A sturdy pyramid structure of TTs extremely enhanced the capture efficiency of targets. The targets triggered the one-step isothermal multi-tandem amplification reaction by incubating multiple hairpin assemblies. To our knowledge, a combination of two parts, which greatly reduced background interference and decreased non-specific substance interference, has appeared for the first time in this paper. Moreover, the load area of electrochemical substances was significantly increased than that in previous studies. This greatly increased the detection range and detection limit of targets. The electrochemical signal responses were generated in freely diffusing hexaammineruthenium(iii) chloride (RuHex). RuHex could adhere to the DNA phosphate backbone by a powerful electrostatic attraction, causing increased current responses."

基金机构：Key Project of Science and Technology Research Program of Chongqing Education Commission [KJZD-K202000404]; Science and Technology Research Program of Chongqing Yuzhong District Science Technology Commission [20180127]

基金资助正文："This work was supported by the Key Project of Science and Technology Research Program of Chongqing Education Commission (grant No. KJZD-K202000404), and the Science and Technology Research Program of Chongqing Yuzhong District Science Technology Commission (grant No. 20180127)."