The establishment of an immunosensor for the detection of SPOP

作者全名："Yue, Song; Sun, Kexin; Li, Siyuan; Liu, Yi; Zhu, Qihao; Chen, Yiyu; Yuan, Dong; Wen, Tao; Ge, Mingjian; Yu, Qiubo"

作者地址："[Yue, Song; Li, Siyuan; Liu, Yi; Zhu, Qihao; Chen, Yiyu; Yuan, Dong; Wen, Tao; Yu, Qiubo] Chongqing Med Univ, Inst Life Sci, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China; [Sun, Kexin] Chongqing Med Univ, Chongqing Eye Inst, Affiliated Hosp 1, Dept Ophthalmol,Chongqing Key Lab Ophthalmol, Chongqing 400016, Peoples R China; [Ge, Mingjian] Chongqing Med Univ, Dept Thorac Surg, Affiliated Hosp 1, Chongqing 400016, Peoples R China"

通信作者："Yu, QB (corresponding author), Chongqing Med Univ, Inst Life Sci, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China.; Ge, MJ (corresponding author), Chongqing Med Univ, Dept Thorac Surg, Affiliated Hosp 1, Chongqing 400016, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:000664660600003

JCR分区：Q1

影响因子：4.6

年份：2021

卷号：11

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："In this paper, we first synthesis three-dimensional jasmine-like Cu@L-aspartic acid(L-ASP) inorganic-organic hybrid nanoflowers to load palladium-platinum nanoparticles (Pd-Pt NPs) as the signal enhancer in order to quantify intracellular speckle-type POZ domain protein. Scanning electron microscope, fourier transform infrared, energy dispersive spectrometer, X-ray photoelectron spectroscopy analysis was used to characterize the newly synthesized materials. The newly formed Cu@L-Asp/Pd-PtNPs can catalyze the decomposition of hydrogen peroxide and exhibit excellent catalytic performance. When different concentration of speckle-type POZ domain protein is captured by speckle-type POZ domain protein antibody linked to the surface of Cu@L-Asp/Pd-Pt NPs, the current signal decreases with the increase concentration of speckle-type POZ domain protein. After optimization, the speckle-type POZ domain protein immunosensor exhibited a good linear response over a concentration range from 0.1-1 ng mL(-1) with a low detection limit of 19 fg mL(-1). The proposed sensor demonstrates good stability within 28 days, acceptable reproducibility (RSD=0.52%) and selectivity to the speckle-type POZ domain protein in the presence of possible interfering substances and has potential application for detecting other intracellular macromolecular substances."

基金机构：Chongqing Yuzhong District of Science and Technology Commission Project [cstc2015shmszx0410]

基金资助正文：This study was financially supported by the Chongqing Yuzhong District of Science and Technology Commission Project (cstc2015shmszx0410) for supporting this project. We would like to express our heartfelt gratitude to all those who have helped us in the course of this project.