Rictor/mTORC2 is involved in endometrial receptivity by regulating epithelial remodeling

作者全名："Zhang, Yue; Du, Xinman; Chen, Xuemei; Tang, Hongyu; Zhou, Qin; He, Junlin; Ding, Yubin; Wang, Yingxiong; Liu, Xueqing; Geng, Yanqing"

作者地址："[Zhang, Yue; Du, Xinman; Chen, Xuemei; Tang, Hongyu; He, Junlin; Ding, Yubin; Wang, Yingxiong; Liu, Xueqing; Geng, Yanqing] Chongqing Med Univ, Sch Publ Hlth & Management, Joint Int Res Lab Reprod & Dev, Chongqing 400016, Peoples R China; [Zhou, Qin] Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Chongqing, Peoples R China; [Wang, Yingxiong; Geng, Yanqing] Chongqing Med Univ, Coll Basic Med, Chongqing, Peoples R China"

通信作者："Liu, XQ; Geng, YQ (corresponding author), Chongqing Med Univ, Sch Publ Hlth & Management, Joint Int Res Lab Reprod & Dev, Chongqing 400016, Peoples R China."

来源：FASEB JOURNAL

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000667814100075

JCR分区：Q1

影响因子：4.8

年份：2021

卷号：35

期号：7

开始页：　

结束页：　

文献类型：Article

关键词：endometrial receptivity; epithelium remodeling; infertility; Na+ channel function; Rictor

摘要："Successful embryo implantation requires well-functioning endometrial luminal epithelial cells to establish uterine receptivity. Inadequate uterine receptivity is responsible for approximately two thirds of implantation failures in humans. However, the regulatory mechanism governing this functional process remains largely unexplored. A previous study revealed that the expression of Rictor, the main member of mTORC2, in mouse epithelial cells is increased on the fourth day of gestation (D4). Here, we provide the first report of the involvement of Rictor in the regulation of endometrial receptivity. Rictor was conditionally ablated in the mouse endometrium using a progesterone receptor cre (PRcre) mouse model. Loss of Rictor altered polarity remodeling and the Na+ channel protein of endometrial cells by mediating Rac-1/PAK1(pPAK1)/ERM(pERM) and Sgk1/pSgk1 signaling, respectively, ultimately resulting in impaired fertility. In the endometrium of women with infertility, the expression of Rictor was changed, along with the morphological transformation and Na+ channel protein of epithelial cells. Our findings demonstrate that Rictor is crucial for the establishment of uterine receptivity in both mice and humans. The present study may help improve the molecular regulatory network of endometrial receptivity and provide new diagnostic and treatment strategies for infertility."

基金机构：National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31801247]

基金资助正文："National Natural Science Foundation of China (NSFC), Grant/Award Number: 31801247"