"A sample-preparation-free, automated, sample-to-answer system for cell counting in human body fluids"

作者全名："Lu, Qiang; Chu, Kaiqin; Dou, Hu; Smith, Zachary J."

作者地址："[Lu, Qiang; Chu, Kaiqin; Smith, Zachary J.] Univ Sci & Technol China, Dept Precis Machinery & Precis Instrumentat, Key Lab Precis Sci Instrumentat, Anhui Higher Educ Inst, 96 Jinzhai Rd, Hefei 230027, Anhui, Peoples R China; [Chu, Kaiqin] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, 96 Jinzhai Rd, Hefei 230027, Anhui, Peoples R China; [Dou, Hu] Chongqing Med Univ, Childrens Hosp, Dept Clin Lab,Chongqing Int Sci & Technol Coopera, Minist Educ,Key Lab Child Dev & Disorders,Key Lab, 136 Zhongshan 2nd Rd, Chongqing 400014, Peoples R China"

通信作者："Smith, ZJ (corresponding author), Univ Sci & Technol China, Dept Precis Machinery & Precis Instrumentat, Key Lab Precis Sci Instrumentat, Anhui Higher Educ Inst, 96 Jinzhai Rd, Hefei 230027, Anhui, Peoples R China.; Dou, H (corresponding author), Chongqing Med Univ, Childrens Hosp, Dept Clin Lab,Chongqing Int Sci & Technol Coopera, Minist Educ,Key Lab Child Dev & Disorders,Key Lab, 136 Zhongshan 2nd Rd, Chongqing 400014, Peoples R China."

来源：ANALYTICAL AND BIOANALYTICAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:000668258800003

JCR分区：Q1

影响因子：4.3

年份：2021

卷号：413

期号：20

开始页：5025

结束页：5035

文献类型：Article

关键词：Clinical/biomedical analysis; Microscopic imaging; Point of care testing; Cell counting

摘要："While many clinical laboratory tests are now highly automated, body fluid cell counting, particularly in low-cellularity samples such as cerebral spinal fluid (CSF), is often performed manually. Here, we report a simple, cost-effective method to obtain white and red blood cell counts from human body fluids such as CSF. The method consists of a compact, automated, and low-cost fluorescence microscope system, coupled to a sample chamber containing all of the necessary reagents in dry form to stain and prepare the sample. Sample focus and scanning are handled automatically, and the acquired multimodal images are automatically analyzed to extract cell counts. Comparison with manual counting on over 200 clinical samples shows excellent agreement. As the system counts a substantially larger image region than a standard manual cell count, we find our sensitivity to extremely low cellularity samples to potentially be higher than the manual gold standard, evidenced by our system recording images of cells in samples whose cell count was registered as ""0"" by a trained user. Thus, our system holds promise for routine, automated, and sensitive analysis of body fluids whose cellularity extends across a wide dynamic range."

基金机构：Ministry of Science and Technology of China's National Key Research and Development Program [2016YFA0201300]; Chongqing Municipal Science and Technology Commission [cstc2017shmsA130083]

基金资助正文："This research was funded by the Ministry of Science and Technology of China's National Key Research and Development Program, Grant number 2016YFA0201300 (ZJS) and the Chongqing Municipal Science and Technology Commission, grant number cstc2017shmsA130083 (HD, ZJS), whose support is gratefully acknowledged."