Gestational diabetes mellitus-associated changes in the breast milk metabolome alters the neonatal growth trajectory

作者全名："Wu, Yue; Yu, Jiaxiao; Liu, Xiyao; Wang, Wenling; Chen, Zhi; Qiao, Juan; Liu, Xiaohui; Jin, Huili; Li, Xin; Wen, Li; Tian, Jing; Saffery, Richard; Kilby, Mark D.; Qi, Hongbo; Tong, Chao; Baker, Philip N."

作者地址："[Wu, Yue; Yu, Jiaxiao; Liu, Xiyao; Chen, Zhi; Qiao, Juan; Jin, Huili; Li, Xin; Wen, Li; Qi, Hongbo; Tong, Chao] Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Wu, Yue; Yu, Jiaxiao; Liu, Xiyao; Chen, Zhi; Qiao, Juan; Jin, Huili; Li, Xin; Wen, Li; Qi, Hongbo; Tong, Chao] Chongqing Med Univ, State Key Lab Maternal & Fetal Med Chongqing Muni, Chongqing 400016, Peoples R China; [Wu, Yue; Yu, Jiaxiao; Liu, Xiyao; Chen, Zhi; Qiao, Juan; Jin, Huili; Li, Xin; Wen, Li; Saffery, Richard; Qi, Hongbo; Tong, Chao; Baker, Philip N.] Chongqing Med Univ, Int Collaborat Lab Reprod & Dev Chinese, Minist Educ, Chongqing 400016, Peoples R China; [Wang, Wenling] Gansu Prov Matern & Child Care Hosp, Dept Obstet, Lanzhou 730050, Gansu, Peoples R China; [Liu, Xiaohui] Tsinghua Univ, Natl Prot Sci Technol Ctr, Beijing 100084, Peoples R China; [Tian, Jing] Chongqing Med Univ, Dept Obstet & Gynecol, Univ Town Hosp, Chongqing 401331, Peoples R China; [Saffery, Richard] Murdoch Childrens Res Inst, Canc Dis & Dev Epigenet, Parkville, Vic 3052, Australia; [Kilby, Mark D.] Univ Birmingham, Coll Med & Dent Sci, Birmingham B15 2TT, W Midlands, England; [Kilby, Mark D.] Birmingham Womens & Childrens Fdn Trust, Fetal Med Ctr, Birmingham B15 2TG, W Midlands, England; [Baker, Philip N.] Univ Leicester, Coll Life Sci, Leicester LE1 7RH, Leics, England"

通信作者："Qi, HB; Tong, C (corresponding author), Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：CLINICAL NUTRITION

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000668712000001

JCR分区：Q1

影响因子：6.3

年份：2021

卷号：40

期号：6

开始页：4043

结束页：4054

文献类型：Article

关键词：Gestational diabetes mellitus; Human breast milk; Metabolome; Neonatal growth

摘要："Background: Gestational diabetes mellitus (GDM) is the most common metabolic disturbance during pregnancy and leads to an altered metabolic profile of human breast milk (HBM). The association between HBM metabolites and neonatal growth in GDM pregnancies has not been thoroughly investigated. Aims: The primary aim was to quantify differences in the HBM metabolome between normal and GDM pregnancies. The secondary aim was to identify metabolites associated with neonatal growth during the first year postpartum. Methods: In the present study, mothers intending to exclusively breastfeed (BF) and their newborns (mother-infant pairs) were recruited at delivery (n = 129 normal pregnancies and n = 98 GDM pregnancies). HBM samples (colostrum, transition milk, and mature milk) from mothers with normal pregnancies (n = 50) and GDM pregnancies (n = 50) were subjected to metabolomic profiling via liquid chromatography tandem mass spectrometry (LC-MS/MS). Receiver operating characteristic (ROC) analysis revealed the metabolomic fingerprints of GDM-associated mature HBM. Correlations between metabolites and neonatal body weight gain (BWG) were evaluated by Spearman correlation analysis. Results: In total, 620 metabolites were identified in each HBM sample; 253 compounds had the same variation patterns, whereas 38 compounds had significantly different pattern transitions between the GDM and normal groups. Moreover, 12, 49 and 28 metabolites exhibited significant differences in the 3 milk types between the 2 groups. Twenty-two metabolites were confirmed by ROC analysis as metabolomic fingerprints in the mature BM of GDM patients. Ten compounds were significantly negatively correlated with neonatal growth, and only 2 unsaturated lipids (eicosatrienoic acid (FA 20:3) and lysophosphatidylcholine (LysoPC) (22:6)) were positively correlated with neonatal BWG. Conclusions: GDM is associated with alterations in the HBM metabolome. Only a small subset of compounds are associated with neonatal body weight (BW)."

基金机构："National Key Research and Development Program of China [2018YFC1002901]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81520108013, 81871189, 82071675]; Chongqing Science and Technology CommissionNatural Science Foundation Project of CQ CSTC [cstc2017jcyjBX0045]; NHC Key Laboratory of Birth Defects and Reproductive Health [2018-5]"

基金资助正文："This work is supported by the National Key Research and Development Program of China (2018YFC1002901), National Natural Science Foundation of China (81520108013, 81871189 and 82071675), Chongqing Science and Technology Commission (cstc2017jcyjBX0045), and NHC Key Laboratory of Birth Defects and Reproductive Health (2018-5)."