Hepatoprotective Effect of Lactobacillus plantarum HFY09 on Ethanol-Induced Liver Injury in Mice

作者全名："Gan, Yi; Tong, Jin; Zhou, Xianrong; Long, Xingyao; Pan, Yanni; Liu, Weiwei; Zhao, Xin"

作者地址："[Gan, Yi; Zhou, Xianrong; Long, Xingyao; Pan, Yanni; Zhao, Xin] Chongqing Univ Educ, Chongqing Collaborat Innovat Ctr Funct Food, Chongqing, Peoples R China; [Gan, Yi; Zhou, Xianrong; Long, Xingyao; Pan, Yanni; Zhao, Xin] Chongqing Univ Educ, Chongqing Engn Res Ctr Funct Food, Chongqing, Peoples R China; [Gan, Yi; Zhou, Xianrong; Long, Xingyao; Pan, Yanni; Zhao, Xin] Chongqing Univ Educ, Chongqing Engn Lab Res & Dev Funct Food, Chongqing, Peoples R China; [Tong, Jin] Chongqing Univ Educ, Chongqing Emergency Med Ctr, Dept Gastroenterol & Hepatol, Chongqing, Peoples R China; [Liu, Weiwei] Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing, Peoples R China"

通信作者："Zhao, X (corresponding author), Chongqing Univ Educ, Chongqing Collaborat Innovat Ctr Funct Food, Chongqing, Peoples R China.; Zhao, X (corresponding author), Chongqing Univ Educ, Chongqing Engn Res Ctr Funct Food, Chongqing, Peoples R China.; Zhao, X (corresponding author), Chongqing Univ Educ, Chongqing Engn Lab Res & Dev Funct Food, Chongqing, Peoples R China.; Liu, WW (corresponding author), Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing, Peoples R China."

来源：FRONTIERS IN NUTRITION

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000670577300001

JCR分区：Q2

影响因子：5

年份：2021

卷号：8

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：lactic acid bacteria; alcoholic liver injury; hepatoprotective effect; antioxidant; anti-inflammatory

摘要："Lactobacillus plantarum is a bacterial strain that is used as a probiotic with health-promoting effects. Our study investigated the hepatoprotective effect of Lactobacillus plantarum HFY09 (LP-HFY09) in mice with ethanol-induced liver injury. The protection afforded by LP-HFY09 was evaluated by observing the morphology of hepatic tissue and measuring liver lipid indexes and function indexes, levels of anti-oxidative enzymes, and anti-inebriation enzymes, as well as oxidative metabolism-related gene expression. Gavage administration of LP-HFY09 [1 x 10(9) CFU/kg body weight (bw)] limited the loss of bw, alcohol damage to the liver, and maintained the normal hepatic tissue morphology. Lactobacillus plantarum HFY09 intervention in ethanol-induced mice led to decreases in serum triglyceride (TG), total cholesterol (TC), aspartic transaminase, alanine transaminase, hyaluronidase (HAase), and precollagen III (PC III), and increases in liver alcohol dehydrogenase (ADH), and acetaldehyde dehydrogenase (ALDH). Lactobacillus plantarum HFY09 assisted with alleviating inflammation by elevating the level of interleukin 10 (IL-10) and decreasing the levels of pro-inflammatory factors [IL-6, IL-1 beta, and tumor necrosis factor-alpha (TNF)-alpha]. Lactobacillus plantarum HFY09 significantly elevated hepatic levels of superoxide dismutase (SOD) and glutathione (GSH), and decreased liver malondialdehyde (MDA) from 3.45 to 1.64 nmol/mg protein. Lactobacillus plantarum HFY09 exhibited an overall strong regulatory effect on liver protection when compared to that of commercial Lactobacillus delbrueckii subsp. bulgaricus. The hepatoprotective effect of LP-HFY09 was reflected by the upregulated expression of peroxisome proliferator activated-receptors alpha, SOD1, SOD2, glutathione peroxidase (GSH-Px), nicotinamide adenine dinucleotide phosphate (NADPH), and catalase (CAT), and the downregulated expression of cyclooxygenase-1 (COX1), c-Jun N-terminal kinase (JNK), and extracellular regulated protein kinases (ERK). Administration of LP-HFY09 at a concentration of 1.0 x 10(9) CFU/kg bw could be a potential intervention, for people who frequently consume alcohol."

基金机构：Chongqing University Innovation Research Group Project [CXQTP20033]; Science and Technology Project of Chongqing Education Commission [KJQN202001604]; Scientific and Technological Innovation Project of Construction of Double City Economic Circle in Chengdu-Chongqing Area of Chongqing Education Commission [KJCX2020052]

基金资助正文："This research was funded by Chongqing University Innovation Research Group Project (CXQTP20033), the Science and Technology Project of Chongqing Education Commission (KJQN202001604), and Scientific and Technological Innovation Project of Construction of Double City Economic Circle in Chengdu-Chongqing Area of Chongqing Education Commission (KJCX2020052), China."