Biomineralization improves the stability of a Streptococcus pneumoniae protein vaccine at high temperatures

作者全名："Min, Yajun; Xu, Wenchun; Xiao, Yunju; Xiao, Jiangming; Shu, Zhaoche; Li, Sijie; Zhang, Jinghui; Liu, Yusi; Yin, Yibing; Zhang, Xuemei; Meng, Jiangping"

作者地址："[Min, Yajun; Xu, Wenchun; Xiao, Yunju; Xiao, Jiangming; Shu, Zhaoche; Li, Sijie; Zhang, Jinghui; Yin, Yibing; Zhang, Xuemei] Chongqing Med Univ, Dept Lab Med, Key Lab Diagnost Med Designated, Minist Educ, Chongqing, Peoples R China; [Meng, Jiangping] Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Assisted Reprod Ctr, Chongqing, Peoples R China; [Liu, Yusi] China Med Univ, Dept Lab Med, Affiliated Hosp 1, Shenyang, Peoples R China; [Min, Yajun] Chongqing Med Univ, Dept Nucl Med, Affiliated Hosp 2, Chongqing, Peoples R China"

通信作者："Meng, JP (corresponding author), Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Assisted Reprod Ctr, Chongqing, Peoples R China."

来源：NANOMEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000673735800001

JCR分区：Q2

影响因子：5.5

年份：2021

卷号：16

期号：20

开始页：1747

结束页：1761

文献类型：Article

关键词：biomineralization; nanoparticles; protein vaccine; Streptococcus pneumoniae; thermal stability

摘要："Aim: Protein vaccines have been the focus of research for vaccine development due to their safety record and facile production. Improving the stability of proteins is of great significance to the application of protein vaccines. Materials & methods: Based on the proteins pneumolysin and DnaJ of Streptococcus pneumoniae, biomineralization was carried out to prepare protein nanoparticles, and their thermal stability was tested both in vivo and in vitro. Results: Mineralized nanoparticles were formed successfully and these calcium phosphate-encapsulated proteins were resistant to proteinase K degradation and were thermally stable at high temperatures. The mineralized proteins retained the immunoreactivity of the original proteins. Conclusion: Mineralization technology is an effective means to stabilize protein vaccines, presenting a safe and economical method for vaccine administration."

基金机构："National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81571622, 31700804, 81772153]"

基金资助正文："This work was supported by Projects of the National Natural Science Foundation of China (number 81571622, number 31700804 and number 81772153). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed."