Intestinal Klebsiella pneumoniae infection enhances susceptibility to epileptic seizure which can be reduced by microglia activation

作者全名："Lin, Peijia; Lin, Aolei; Tao, Kaiyan; Yang, Min; Ye, Qinglin; Chen, Hongnian; Chen, Yuanyuan; Ma, Yuanlin; Lin, Zijun; He, Miaoqing; Wang, Xuefeng; Tian, Xin"

作者地址："[Lin, Peijia; Lin, Aolei; Tao, Kaiyan; Yang, Min; Ye, Qinglin; Chen, Hongnian; Chen, Yuanyuan; Ma, Yuanlin; Lin, Zijun; He, Miaoqing; Wang, Xuefeng; Tian, Xin] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurol, Chongqing Key Lab Neurol, Chongqing, Peoples R China"

通信作者："Wang, XF; Tian, X (corresponding author), Chongqing Med Univ, Affiliated Hosp 1, Dept Neurol, Chongqing Key Lab Neurol, Chongqing, Peoples R China."

来源：CELL DEATH DISCOVERY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000674570600002

JCR分区：Q2

影响因子：7

年份：2021

卷号：7

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Epilepsy is a common nervous system disease, and the existing theory does not fully clarify its pathogenesis. Recent research suggests that intestinal microbes may be involved in the development of epilepsy, but which microbe is involved remains unclear. We used 16s rRNA sequencing to identify the most relevant gut microbe. To determine the relationship between this microbe and epilepsy, we used an animal model. In addition, western blotting and immunofluorescence, as well as inhibitor studies, were used to evaluate and confirm the role of microglia in this process. In this study, we first report an increase in gut Klebsiella pneumoniae in patients with epilepsy. Subsequently, animal studies revealed that Klebsiella pneumoniae in the intestinal tract affects seizure susceptibility and activates microglial cells to release inflammatory factors. Furthermore, the inflammatory response of microglial cells plays a protective role in the seizure susceptibility caused by an increased abundance of Klebsiella pneumoniae. Our results suggest that gut disruption may be involved in seizure regulation and microglia protect the brain against seizure under this condition. These findings provide a new perspective for research on the pathogenesis and prevention of epilepsy."

基金机构：National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [82001378]; Chongqing Postgraduate Research Innovation Project [CYB19143]

基金资助正文："Authors wish to acknowledge all the participants and technicians participate in this study. This work was supported by grants from the National Natural Science Foundation of China (grant number 82001378 to Xin Tian), Chongqing Postgraduate Research Innovation Project (CYB19143 to Peijia Lin)."