Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-Renewal

作者全名："He, Jianrong; Cheng, Yuda; Ruan, Yan; Wang, Jiali; Tian, Yanping; Wang, Jiaqi; Wang, Fengsheng; Zhang, Chen; Xu, Yixiao; Liu, Lianlian; Yu, Meng; Wang, Jiangjun; Zhao, Binyu; Zhang, Yue; Yang, Yi; Liu, Gaoke; Wu, Wei; He, Ping; Xiong, Jiaxiang; Huang, He; Zhang, Junlei; Jian, Rui"

作者地址："[He, Jianrong; Huang, He] Chongqing Med Univ, Affiliated Hosp 2, Dept Anesthesiol, Chongqing 400010, Peoples R China; [He, Jianrong; Cheng, Yuda; Ruan, Yan; Wang, Jiali; Tian, Yanping; Wang, Jiaqi; Wang, Fengsheng; Zhang, Chen; Xu, Yixiao; Liu, Lianlian; Yu, Meng; Wang, Jiangjun; Zhao, Binyu; Zhang, Yue; Liu, Gaoke; Zhang, Junlei; Jian, Rui] Army Med Univ, Lab Stem Cell & Dev Biol, Dept Histol & Embryol, Chongqing 400038, Peoples R China; [Xu, Yixiao; Zhang, Yue] Army Med Univ, Hosp Affiliated 1, Southwest Eye Hosp, Southwest Hosp, Chongqing 400038, Peoples R China; [Yang, Yi; Xiong, Jiaxiang] Army Med Univ, Coll Basic Med Sci, Expt Ctr Basic Med, Chongqing 400038, Peoples R China; [Wu, Wei] Army Med Univ, Hosp Affiliated 1, Southwest Hosp, Thorac Surg Dept, Chongqing 400038, Peoples R China; [He, Ping] Army Med Univ, Hosp Affiliated 1, Southwest Hosp, Cardiac Surg Dept, Chongqing 400038, Peoples R China"

通信作者："Huang, H (corresponding author), Chongqing Med Univ, Affiliated Hosp 2, Dept Anesthesiol, Chongqing 400010, Peoples R China.; Zhang, JL (corresponding author), Army Med Univ, Lab Stem Cell & Dev Biol, Dept Histol & Embryol, Chongqing 400038, Peoples R China."

来源：STEM CELLS INTERNATIONAL

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000675692700001

JCR分区：Q3

影响因子：4.3

年份：2021

卷号：2021

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Dax1(also known as Nr0b1) is regarded as an important component of the transcription factor network in mouse embryonic stem cells (ESCs). However, the role and the molecular mechanism of Dax1 in the maintenance of different pluripotency states are poorly understood. Here, we constructed a stable Dax1 knockout (KO) cell line using the CRISPR/Cas9 system to analyze the precise function of Dax1. We reported that 2i/LIF-ESCs had significantly lower Dax1 expression than LIF/serum-ESCs. Dax1KO ES cell lines could be established in 2i/LIF and their pluripotency was confirmed. In contrast, Dax1-null ESCs could not be continuously passaged in LIF/serum due to severe differentiation and apoptosis. In LIF/serum, the activities of the Core module and Myc module were significantly reduced, while the PRC2 module was activated after Dax1KO. The expression of most proapoptotic genes and lineage-commitment genes were drastically increased, while the downregulated expression of antiapoptotic genes and many pluripotency genes was observed. Our research on the pluripotent state-dependent role of Dax1 provides clues to understand the molecular regulation mechanism at different stages of early embryonic development."

基金机构："National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31571442, 31601108, 81702294, 81572830, 81572885]"

基金资助正文："This work was supported in part by grants from the National Natural Science Foundation of China (31571442, 31601108, 81702294, 81572830, 81572885)."