Porous matrix nanometric scaffold repairs rabbit tibia injury by promoting bone marrow mesenchymal stem cell osteogenic differentiation and up-regulating type I and II collagen content

作者全名："Dong, Bozan; Zhang, Dongjie"

作者地址："[Dong, Bozan] Chongqing Med Univ, Dept Ctr Orthoped Trauma, Affiliated Hosp 3, Chongqing 400000, Peoples R China; [Zhang, Dongjie] Bishan Hosp Chongqing, Dept Orthoped, Chongqing 400000, Peoples R China"

通信作者："Zhang, DJ (corresponding author), Bishan Hosp Chongqing, Dept Orthoped, Chongqing 400000, Peoples R China."

来源：MATERIALS EXPRESS

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000675725600003

JCR分区：Q4

影响因子：0.7

年份：2021

卷号：11

期号：7

开始页：1031

结束页：1037

文献类型：Article

关键词：Porous Nano-Matrix; Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cell; Rabbit Tibia Injury; Collagen Type I and II Content

摘要："To explore the effect of porous matrix nanometer scaffold on Bone marrow mesenchymal stem cell osteogenic differentiation and type I and II collagen content in rabbit tibial injury. 22 rabbits, weighing 2.20-3.10 kg, were equally divided into model and treatment groups. Von Kossa was used to detect osteogenic differentiation, while Western blot detected type I and type II collagen. The bone density meter measurement software was used to assess tibial bone density. Universal material testing machine was used to detect biomechanical indicators level, while HE staining detected bone morphology. Adherent Cells growth were observed in a week; where cells clusters appeared and continued to grow 2 weeks later, and the number of osteoblasts and mineralized nodules increased significantly 3 weeks later. The collagen level in the treatment group was significantly higher than that in model group (P < 0.05). The tibia bone mineral density in model group was significantly lower at the sixth and twelfth weeks after surgery (P < 0.05). The maximum bending load and bending stiffness of model group were significantly lower than those of treatment group with higher damage degree (P < 0.05). Treatment increased the bone tissue and osteoblasts, leading to production of trabecular bone structure. No clustered regenerated bone-like tissues in model group were observed. The porous matrix nanometer scaffold can repair rabbit tibia injury possibly by promoting Bone marrow mesenchymal stem cell osteogenic differentiation and increasing type I and type II collagen level."

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