Characterization of an Enterococcus faecalis Bacteriophage vB_EfaM_LG1 and Its Synergistic Effect With Antibiotic

作者全名："Song, Min; Wu, Dongmei; Hu, Yang; Luo, Haiyan; Li, Gongbo"

作者地址："[Song, Min; Wu, Dongmei; Hu, Yang; Luo, Haiyan; Li, Gongbo] Chongqing Med Univ, Dept Neurol, Affiliated Hosp 2, Chongqing, Peoples R China"

通信作者："Li, GB (corresponding author), Chongqing Med Univ, Dept Neurol, Affiliated Hosp 2, Chongqing, Peoples R China."

来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000679818200001

JCR分区：Q2

影响因子：5.7

年份：2021

卷号：11

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：bacteriophage; phage-antibiotic combination; Enterococcus faecalis; antibiotic resistance; phage therapy

摘要："Enterococcus faecalis is a Gram-positive opportunistic pathogen that could cause pneumonia and bacteremia in stroke patients. The development of antibiotic resistance in hospital-associated E. faecalis is a formidable public health threat. Bacteriophage therapy is a renewed solution to treat antibiotic-resistant bacterial infections. However, bacteria can acquire phage resistance quite quickly, which is a significant barrier to phage therapy. Here, we characterized a lytic E. faecalis bacteriophage Vb_EfaM_LG1 with lytic activity. Its genome did not contain antibiotic resistance or virulence genes. Vb_EfaM_LG1 effectively inhibits E. faecalis growth for a short period, and phage resistance developed within hours. However, the combination of antibiotics and phage has a tremendous synergistic effect against E. faecalis, prevents the development of phage resistance, and disrupts the biofilm efficiently. Our results show that the phage-antibiotic combination has better killing efficiency against E. faecalis."

基金机构：Chongqing Natural Science FoundationNatural Science Foundation of Chongqing [cstc2019jcyj-msxmX0173]

基金资助正文：This work was supported by Chongqing Natural Science Foundation (cstc2019jcyj-msxmX0173).