Circular RNA circSP3 promotes hepatocellular carcinoma growth by sponging microRNA-198 and upregulating cyclin-dependent kinase 4

作者全名："Li, Molin; Chen, Hang; Xia, Lulu; Huang, Ping"

作者地址："[Li, Molin; Huang, Ping] Chongqing Med Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Chongqing 400000, Peoples R China; [Chen, Hang] Peoples Hosp Tongliang Dist, Dept Hematol & Oncol, Chongqing 402560, Peoples R China; [Xia, Lulu] Chongqing Med Univ, Coll Lab Med, Chongqing 400042, Peoples R China"

通信作者："Huang, P (corresponding author), Chongqing Med Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Chongqing 400000, Peoples R China."

来源：AGING-US

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000680282000002

JCR分区：Q2

影响因子：5.2

年份：2021

卷号：13

期号：14

开始页：18586

结束页：18605

文献类型：Article

关键词：circSP3; miR-198; HCC; proliferation; migration

摘要："As a new class of endogenous noncoding RNAs, circular RNAs (circRNAs), have been found to influence cell development and function by sponging microRNAs. MicroRNA (miR)-198 is downregulated in various cancers, including hepatocellular carcinoma (HCC). We therefore searched for dysregulated circRNAs that could sponge miR-198 in HCC. By analyzing relevant circRNA databases (circBase, TargetScan and CircInteractome), we found that the miR-198-binding circRNA hsa_circSP3 is upregulated in HCC. CircSP3 expression correlated negatively with miR-198 expression in HCC tissues. Dual luciferase reporter assays indicated that circSP3 bound to miR-198. CircSP3 overexpression in HCC cells induced expression of cyclin-dependent kinase 4, a target gene of miR-198. Silencing circSP3 inhibited HCC cell proliferation and migration by downregulating cyclin-dependent kinase 4, whereas inhibiting miR-198 reversed those effects. In vivo experiments confirmed that circSP3 promoted xenograft tumor growth. These data suggest that circSP3 may be a novel biomarker for HCC."

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