Altered Fecal Metabolites and Colonic Glycerophospholipids Were Associated With Abnormal Composition of Gut Microbiota in a Depression Model of Mice

作者全名："Gong, Xue; Huang, Cheng; Yang, Xun; Chen, Jianjun; Pu, Juncai; He, Yong; Xie, Peng"

作者地址："[Gong, Xue; Yang, Xun; Pu, Juncai; He, Yong; Xie, Peng] Chongqing Med Univ, Affiliated Hosp 1, NHC Key Lab Diag & Treatment Brain Funct Dis, Chongqing, Peoples R China; [Gong, Xue; Yang, Xun; Pu, Juncai; He, Yong; Xie, Peng] Chongqing Med Univ, Dept Neurol, Affiliated Hosp 1, Chongqing, Peoples R China; [Gong, Xue; Pu, Juncai; He, Yong; Xie, Peng] Chongqing Key Lab Neurobiol, Chongqing, Peoples R China; [Huang, Cheng] Jinan Univ, Clin Neurosci Inst, Affiliated Hosp 1, Dept Neurol, Guangzhou, Peoples R China; [Huang, Cheng] Jinan Univ, Clin Neurosci Inst, Affiliated Hosp 1, Stroke Ctr, Guangzhou, Peoples R China; [Chen, Jianjun] Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China"

通信作者："Xie, P (corresponding author), Chongqing Med Univ, Affiliated Hosp 1, NHC Key Lab Diag & Treatment Brain Funct Dis, Chongqing, Peoples R China.; Xie, P (corresponding author), Chongqing Med Univ, Dept Neurol, Affiliated Hosp 1, Chongqing, Peoples R China.; Xie, P (corresponding author), Chongqing Key Lab Neurobiol, Chongqing, Peoples R China."

来源：FRONTIERS IN NEUROSCIENCE

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000680355500001

JCR分区：Q2

影响因子：4.3

年份：2021

卷号：15

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：microbiota-gut-brain axis; chronic social defeated stress; gut microbiota; fecal metabolome; colonic lipids; glycerophospholipids

摘要："The microbiota-gut-brain axis has been considered to play an important role in the development of depression, but the underlying mechanism remains unclear. The gastrointestinal tract is home to trillions of microbiota and the colon is considered an important site for the interaction between microbiota and host, but few studies have been conducted to evaluate the alterations in the colon. Accordingly, in this study, we established a chronic social defeated stress (CSDS) mice model of depression. We applied 16S rRNA gene sequencing to assess the gut microbial composition and gas and liquid chromatography-mass spectroscopy to identify fecal metabolites and colonic lipids, respectively. Meanwhile, we used Spearman's correlation analysis method to evaluate the associations between the gut microbiota, fecal metabolites, colonic lipids, and behavioral index. In total, there were 20 bacterial taxa and 18 bacterial taxa significantly increased and decreased, respectively, in the CSDS mice. Further, microbial functional prediction demonstrated a disturbance of lipid, carbohydrate, and amino acid metabolism in the CSDS mice. We also found 20 differential fecal metabolites and 36 differential colonic lipids (in the category of glycerolipids, glycerophospholipids, and sphingolipids) in the CSDS mice. Moreover, correlation analysis showed that fecal metabolomic signature was associated with the alterations in the gut microbiota composition and colonic lipidomic profile. Of note, three lipids [PC(16:0/20:4), PG(22:6/22:6), and PI(18:0/20:3), all in the category of glycerophospholipids] were significantly associated with anxiety- and depression-like phenotypes in mice. Taken together, our results indicated that the gut microbiota might be involved in the pathogenesis of depression via influencing fecal metabolites and colonic glycerophospholipid metabolism."

基金机构：National Key Research and Development Program of China [2017YFA0505700]; Non-Profit Central Research Institute Fund of Chinese Academy of Medical Sciences [2019PT320002300]; Natural Science Foundation Project of ChinaNational Natural Science Foundation of China (NSFC) [81820108015]

基金资助正文："This work was supported by the National Key Research and Development Program of China (Grant No. 2017YFA0505700), the Non-Profit Central Research Institute Fund of Chinese Academy of Medical Sciences (Grant No. 2019PT320002300), and the Natural Science Foundation Project of China (Grant No. 81820108015)."