Ultrasonic Sputter Desorption Mass Spectrometry Technique for Minimally Invasive Tissue Analysis

作者全名："Fu, Xian; Wang, Yu; Xia, Bing; Shi, Peiyu; Zhou, Yan"

作者地址："[Fu, Xian; Wang, Yu; Xia, Bing; Shi, Peiyu; Zhou, Yan] Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China; [Fu, Xian] Chongqing Med Univ, Inst Life Sci, Ctr Novel Target & Therapeut Intervent, Chongqing 400016, Peoples R China; [Wang, Yu; Shi, Peiyu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China"

通信作者："Xia, B; Zhou, Y (corresponding author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China."

来源：ANALYTICAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:000683353300016

JCR分区：Q1

影响因子：7.4

年份：2021

卷号：93

期号：30

开始页：10502

结束页：10510

文献类型：Article

关键词：　

摘要："Minimally invasive techniques for in vivo tissue analysis are desired by life science and medical research. Herein, a new ionization interface coupled with ultrasonic sputter desorption (USD) was developed for in vitro and in vivo tissue analysis. Sample molecules were effectively sputtered out when the high-frequency ultrasonic probe touched the tissue. Then, the sputtered molecules were collected and ionized by a custom-made heated quartz tube and finally analyzed by mass spectrometry (MS) online. The sample pretreatment of the USD-MS technique was quite simple and required no other steps except for wetting the tissue surface with ethanol to assist molecular extraction. Experimental results demonstrated that the proposed method was suitable for the analysis of different morphologies of tissues (such as liver, brain, kidney, and lung) and performed well in the analysis of liver tumors and paracancerous tissues. Moreover, as the proposed method caused little damage to the tissues during analysis, rats and mice with orthotopic tumors still survived after the experiments. Overall, the newly developed USD-MS technique was an effective tool for minimally invasive tissue analysis and could be used as a new candidate method for in situ and real-time analysis of biological tissues in vitro and in vivo."

基金机构："National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21772193]; Scientific Research Equipment Development Project of CAS [YJKYYQ20170001]; Youth Innovation Promotion Association, CAS [2019361]"

基金资助正文："This research was supported by the National Natural Science Foundation of China (21772193), the Scientific Research Equipment Development Project of CAS (no. YJKYYQ20170001), and the Youth Innovation Promotion Association, CAS (2019361)."