Establishing a three-miRNA signature as a prognostic model for colorectal cancer through bioinformatics analysis

作者全名："Wang, Yiming; Huang, Lumi; Shan, Nan; Ma, Huiwen; Lu, Songmei; Chen, Xingyue; Long, Hao"

作者地址："[Wang, Yiming; Huang, Lumi; Ma, Huiwen; Lu, Songmei; Chen, Xingyue] Chongqing Univ, Canc Hosp, Dept Med Oncol, Chongqing, Peoples R China; [Shan, Nan] Chongqing Med Univ, Affiliated Hosp 1, Dept Gynaecol & Obstet, Chongqing, Peoples R China; [Long, Hao] Chongqing Univ, Canc Hosp, Dept Palliat Care, Chongqing, Peoples R China"

通信作者："Long, H (corresponding author), Chongqing Univ, Canc Hosp, Dept Palliat Care, Chongqing, Peoples R China."

来源：AGING-US

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000685261000012

JCR分区：Q2

影响因子：5.2

年份：2021

卷号：13

期号：15

开始页：19894

结束页：19907

文献类型：Article

关键词：CCa; miRNA; risk score; prognostic model

摘要："Background: Identification of more promising microRNAs (miRNAs) are being extensively studied with respect to colorectal cancer (CRC), since CRC is the leading cause of cancer deaths and most common malignant tumors worldwide. A series of colon cancer (CCa) samples from The Cancer Genome Atlas (TCGA) were analyzed to provide a new perspective into this field. Methods: The expression of miRNAs, mRNAs and the clinical data of 437 CRC patients were downloaded from the TCGA database. The survival-related differentially expressed miRNAs (sDMIRs) and mRNAs were detected by COX regression analysis. The high-risk group and low-risk group were separated by the median risk score of the risk score model. The potential clinical characteristics of these sDMIRs were analyzed by R software. The potential molecular mechanisms of these sDMIRs were explored by computational biology. The expression levels of three sDMIRs were explored by qPCR in CRC samples. Results: Three DMIRs (hsa-miR-21-3p, hsa-miR-194-3p and hsa-miR-891a-5p) correlated with the most remarkable prognostic values of CRC patients were selected to establish the risk score model (RSM) by univariate and multivariate COX regression analysis and the survival probability of the low-risk group was longer than that in the high-risk group. We detected the target genes of three sDMIRs and the potential molecular mechanisms of these sDMIRs. We further verified the high expression levels of hsa-miR-21-3p and hsa-miR-194-3p were associated with the early T-stages, while hsa-miR-891a-5p illustrated the reversed result. Conclusion: Our study demonstrated three sDMIRs with significantly clinical values illustrated the potential predicting values in the prognosis of CRC patients. Our results may provide a new perspective for the diagnostic methods and treatment strategies in CRC patients."

基金机构：Chongqing Science and Technology Bureau [cstc2019jcyjmsxmX0721]

基金资助正文："Chongqing Science and Technology Bureau, cstc2019jcyjmsxmX0721."