CRNDE enhances the expression of MCM5 and proliferation in acute myeloid leukemia KG-1a cells by sponging miR-136-5p

作者全名："Liu, Chen; Zhong, Liang; Shen, Chenlan; Chu, Xuan; Luo, Xu; Yu, Lihua; Ye, Jiao; Xiong, Ling; Dan, Wenran; Li, Jian; Liu, Beizhong"

作者地址："[Liu, Chen; Shen, Chenlan; Chu, Xuan; Luo, Xu; Xiong, Ling; Dan, Wenran; Liu, Beizhong] Chongqing Med Univ, Cent Lab Yong Chuan Hosp, Chongqing 402160, Peoples R China; [Liu, Chen; Zhong, Liang; Ye, Jiao; Li, Jian; Liu, Beizhong] Chongqing Med Univ, Dept Lab Med, Key Lab Lab Med Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Yu, Lihua] Chongqing Med Univ, Clin Lab Yong Chuan Hosp, Chongqing 402160, Peoples R China"

通信作者："Liu, BZ (corresponding author), Chongqing Med Univ, Cent Lab Yong Chuan Hosp, Chongqing 402160, Peoples R China.; Liu, BZ (corresponding author), Chongqing Med Univ, Dept Lab Med, Key Lab Lab Med Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:000686708000035

JCR分区：Q1

影响因子：4.6

年份：2021

卷号：11

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："The long-noncoding RNA colorectal neoplasia differentially expressed (CRNDE) gene has been considered to be crucial in tumor malignancy. Although CRNDE is highly expressed in acute myeloid leukemia (AML), its mechanism of action remains unknown. In this study, GEPIA and qRT-PCR were performed to confirm the expression of CRNDE in AML samples and cell lines, respectively. CRNDE shRNA vectors were transfected to explore the biological functions of CRNDE. The cell proliferation was assessed by the CCK8 assay, while apoptosis and cell cycle distribution were measured by flow cytometry and Western blotting. The results showed that CRNDE was overexpressed in both AML samples and cell lines. CRNDE silencing inhibited proliferation and increased apoptotic rate and cell cycle arrest of KG-1a cells. The luciferase reporter assay coupled with RIP assay revealed that CRNDE act as a ceRNA. Rescue assays demonstrated that the effects of CRNDE silencing could be reversed by miR-136-5p inhibitors. In conclusion, our results expound that the CRNDE/miR-136-5p/MCM5 axis modulates cell progression and provide a new regulatory network of CRNDE in KG-1a cells."

基金机构："National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81772280]; Open Research Fund of the Key Laboratory of Biotherapy, West China Hospital, West China Medicine School, Sichuan University [SKLB202008]"

基金资助正文："This work was supported by grants from the National Natural Science Foundation of China (Grant Number 81772280) and the Open Research Fund of the Key Laboratory of Biotherapy, West China Hospital, West China Medicine School, Sichuan University (Grant Number SKLB202008)."