Exogenous Autoinducer-2 Rescues Intestinal Dysbiosis and Intestinal Inflammation in a Neonatal Mouse Necrotizing Enterocolitis Model

作者全名："Ji, Yan-Chun; Sun, Qian; Fu, Chun-Yan; She, Xiang; Liu, Xiao-Chen; He, Yu; Ai, Qing; Li, Lu-Quan; Wang, Zheng-Li"

作者地址："[Ji, Yan-Chun; Sun, Qian; Fu, Chun-Yan; She, Xiang; Liu, Xiao-Chen; He, Yu; Ai, Qing; Li, Lu-Quan; Wang, Zheng-Li] Chongqing Med Univ, Neonatal Diag & Treatment Ctr, Childrens Hosp, Chongqing, Peoples R China; [Ji, Yan-Chun; Sun, Qian; Fu, Chun-Yan; She, Xiang; Liu, Xiao-Chen; He, Yu; Ai, Qing; Li, Lu-Quan; Wang, Zheng-Li] Natl Clin Res Ctr Chid Hlth & Disorders, Chongqing, Peoples R China; [Ji, Yan-Chun; Sun, Qian; Fu, Chun-Yan; She, Xiang; Liu, Xiao-Chen; He, Yu; Ai, Qing; Li, Lu-Quan; Wang, Zheng-Li] Minist Educ, Key Lab Chid Dev & Disorders, Chongqing, Peoples R China; [Ji, Yan-Chun; Sun, Qian; Fu, Chun-Yan; She, Xiang; Liu, Xiao-Chen; He, Yu; Ai, Qing; Li, Lu-Quan; Wang, Zheng-Li] China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China; [Ji, Yan-Chun; Sun, Qian; Fu, Chun-Yan; She, Xiang; Liu, Xiao-Chen; He, Yu; Ai, Qing; Li, Lu-Quan; Wang, Zheng-Li] Chongqing Key Lab Pediat, Chongqing, Peoples R China"

通信作者："Li, LQ; Wang, ZL (corresponding author), Chongqing Med Univ, Neonatal Diag & Treatment Ctr, Childrens Hosp, Chongqing, Peoples R China.; Li, LQ; Wang, ZL (corresponding author), Natl Clin Res Ctr Chid Hlth & Disorders, Chongqing, Peoples R China.; Li, LQ; Wang, ZL (corresponding author), Minist Educ, Key Lab Chid Dev & Disorders, Chongqing, Peoples R China.; Li, LQ; Wang, ZL (corresponding author), China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China.; Li, LQ; Wang, ZL (corresponding author), Chongqing Key Lab Pediat, Chongqing, Peoples R China."

来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000687646100001

JCR分区：Q2

影响因子：5.7

年份：2021

卷号：11

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：necrotizing enterocolitis (NEC); autoinducer-2 (AI-2); intestinal flora; cytokines; intestinal immunity

摘要："Autoinducer-2 (AI-2) is believed to be a bacterial interspecies signaling molecule that plays an important role in the regulation of the physiological behaviors of bacteria. The effect of AI-2 on the process of necrotizing enterocolitis (NEC) is unknown, and the aim of this study was to study the effect of AI-2 in a mouse NEC model. C57BL/6 mouse pups were randomly divided into three groups: the control group, the NEC group, and the NEC+AI-2 (NA) group. Exogenous AI-2 (500 nM) was added to the formula milk of the NA group. The concentrations of fecal AI-2 and flora were tested. The expression of cytokines, TLR4 and NF-kappa B in intestinal tissue was detected. The AI-2 level was significantly decreased in the NEC group (P<0.05). Compared with the NEC group, the intestinal injury scores, expression of TLR4, NF-kB, and proinflammatory factors (IL-1 beta, IL-6, IL-8 and TNF-alpha) were reduced, and expression of anti-inflammatory factor (IL-10) was increased in the NA group mice (P<0.05). At the phylum level, the Proteobacteria abundance in the NA group was significantly increased, while the Bacteroidota abundance in the control group was significantly increased (P<0.05). At the genus level, Helicobacter and Clostridium_sensu_stricto_1 exhibited significantly greater abundance in the NEC group than in the other two groups, while Lactobacillus had the opposite trend (P<0.05). In addition, the abundances of Klebsiella, Rodentibacter and Enterococcus were significantly higher in the NA group than in the NEC and control groups (P < 0.05). Exogenous AI-2 partially reverses flora disorder and decreases inflammation in an NEC mouse model."

基金机构：Scientific Research Foundation of the Science and Technology Commission of Chongqing [cstc2019jcyj-msxmX0169]; General Basic Research Project from the Ministry of Education Key Laboratory of Child Development and Disorders [GBRP-202107]; Chongqing Municipal Administration of Human Resources and Social Security [Cx2017107]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [82001602]; Science and Health Project of the Chongqing Health Commission [2020FYYX217]

基金资助正文："This work was supported by the Scientific Research Foundation of the Science and Technology Commission of Chongqing (Grant No. cstc2019jcyj-msxmX0169), the General Basic Research Project from the Ministry of Education Key Laboratory of Child Development and Disorders (Grant No. GBRP-202107), the Chongqing Municipal Administration of Human Resources and Social Security (Grant No. Cx2017107), the National Natural Science Foundation of China (Grant No. 82001602), and the Science and Health Project of the Chongqing Health Commission (Grant No. 2020FYYX217)."