An "off-on" electrochemiluminescence biosensor coupled with strand displacement-powered 3D micromolecule walking nanomachine for ultrasensitive detection of adenosine triphosphate

作者全名："Li, Li; Zhou, Zheng; Li, Xinmin; Li, Dandan; Zhao, Min; Liu, Changjin; Wu, Haiping; Yang, Wei; Ding, Shijia; Shen, Bo"

作者地址："[Li, Li] Univ Chinese Acad Sci, Chongqing Gen Hosp, Dept Lab Med, Chongqing 400014, Peoples R China; [Zhou, Zheng] Chongqing Univ, Dept Lab Med, Three Gorges Hosp, Chongqing 404000, Peoples R China; [Li, Xinmin; Li, Dandan; Zhao, Min; Liu, Changjin; Wu, Haiping; Yang, Wei; Ding, Shijia; Shen, Bo] Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Clin Lab Diagnost, Chongqing 400016, Peoples R China; [Li, Xinmin; Shen, Bo] Chongqing Hosp Tradit Chinese Med, Dept Lab Med, Chongqing 400021, Peoples R China"

通信作者："Shen, B (corresponding author), Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Clin Lab Diagnost, Chongqing 400016, Peoples R China.; Shen, B (corresponding author), Chongqing Hosp Tradit Chinese Med, Dept Lab Med, Chongqing 400021, Peoples R China."

来源：MICROCHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000691689400001

JCR分区：Q1

影响因子：5.7

年份：2021

卷号：188

期号：7

开始页：　

结束页：　

文献类型：Article

关键词：Electrochemiluminescence; Biosensor; Nanomachine; Strand displacement; Switching signal; Adenosine triphosphate determination

摘要："A three-dimensional (3D) micromolecule walking nanomachine propelled by strand displacement was developed to establish a novel switching electrochemiluminescence (ECL) biosensor for ultrasensitive detection of adenosine triphosphate (ATP). Generally, walking nanomachines reported previously were limited to DNA walkers, while the proposed 3D walking nanomachine focused on the micromolecule walker. Firstly, TiO2 and silver nanoparticles (Ag NPs) functionalized N-(4-aminobutyl)-N-(ethylisoluminol) (Ag-ABEI) were deposited onto the electrode surface to offer an enhanced ECL signal, resulting from the double catalytic effect of TiO2 and Ag NPs for H2O2. Following, dopamine (DA)-labeled DNA duplex probes (S1/S2-DA) immobilized onto the modified electrode cut down the original ECL signal due to the quenching of DA toward ABEI (signal-off). Target ATP walker moved along the 3D DNA track, simultaneously releasing numerous DNA3, which was applied to displace S2-DA, resulting in the quenched ECL intensity recovery (signal-on). As a result, the biosensor showed a low limit of detection down to 0.5 nM (S/N = 3) and was successfully employed to determine ATP in human serum samples. Thus, the established biosensing strategy holds great potential for biochemical studies and clinical diagnosis."

基金机构："National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [82002257, 81702083]; Natural Science Foundation Project of ChongqingNatural Science Foundation of Chongqing [cstc2020jcyj-msxmX0190]; Science and Technology Plan Project of Yuzhong District of Chongqing [20170128]; Hospital-special Fund of Chengdu University of TCM [YYZX20180030]"

基金资助正文："This work was funded by financial support from the National Natural Science Foundation of China (82002257, 81702083), the Natural Science Foundation Project of Chongqing (cstc2020jcyj-msxmX0190), the Science and Technology Plan Project of Yuzhong District of Chongqing (20170128), and Hospital-special Fund of Chengdu University of TCM (YYZX20180030)."