One-Pot Identification of BCR/ABL(p210) Transcript Isoforms Based on Nanocluster Beacon

作者全名："Gou, Xiaolong; Xu, Lulu; Yang, Suqing; Cheng, Xiaoxue; Wu, Haiping; Zhang, Decai; Shi, Weicheng; Ding, Shijia; Zhang, Yuhong; Cheng, Wei"

作者地址："[Gou, Xiaolong; Xu, Lulu; Cheng, Xiaoxue; Zhang, Decai; Shi, Weicheng; Zhang, Yuhong; Cheng, Wei] Chongqing Med Univ, Ctr Clin Mol Med Detect, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Xu, Lulu] Shandong First Med Univ, Dept Clin Lab, Shandong Prov Hosp, Jinan 250021, Shandong, Peoples R China; [Yang, Suqing] Chongqing Testing & Lnspect Ctr Med Devices, Chongqing 400016, Peoples R China; [Wu, Haiping; Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Clin Lab Diagnost, Chongqing 400016, Peoples R China"

通信作者："Zhang, YH; Cheng, W (corresponding author), Chongqing Med Univ, Ctr Clin Mol Med Detect, Affiliated Hosp 1, Chongqing 400016, Peoples R China."

来源：ACS SENSORS

ESI学科分类：CHEMISTRY

WOS号：WOS:000692037700017

JCR分区：Q1

影响因子：8.9

年份：2021

卷号：6

期号：8

开始页：2928

结束页：2937

文献类型：Article

关键词：biosensor; AgNCs; fluorescence assay; BCR/ABL(p210) fusion gene; chronic myeloid leukemia

摘要："The BCR/ABL(p210) fusion gene is a classic biomarker of chronic myeloid leukemia, which can be divided into e13a2 and e14a2 isoforms according to different breakpoints. These two isoforms showed distinct differences in clinical manifestation, treatment effect, and prognosis risk. Herein, a strategy based on nanocluster beacon (NCB) fluorescence was developed to identify the e13a2 and e14a2 isoforms in one-pot. Because the fluorescence of AgNCs can be activated when they are placed in proximity to the corresponding enhancer sequences, thymine-rich (T-rich) or guanine-rich (G-rich). In this work, we explored an ideal DNA-AgNCs template as an excellent molecular reporter with a high signal-to-noise ratio. After recognition with the corresponding isoforms, the AgNCs can be pulled closer to the T-rich or G-rich sequences to form a three-way junction structure and generate fluorescence with corresponding wavelengths. Therefore, by distinguishing the corresponding wavelengths of AgNCs, we successfully identified two isoforms in one tube with the limitation of 16 pM for e13a2 and 9 pM for e14a2. Moreover, this strategy also realized isoform identification in leukemia cells and newly diagnosed CML patients within 40 min, which provides a powerful tool to distinguish fusion gene subtypes at the same time."

基金机构："National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81572080, 81873972]; Natural Science Foundation Project of ChongqingNatural Science Foundation of Chongqing [cstc2015shmszx120086]; Training Program for Advanced Young Medical Personnel of Chongqing [2017HBRC003]; Chongqing Science Fund for Distinguished Young Scholars [cstc2019jcyjjqX0028]; Foundation for Innovative Research Groups of Chongqing Higher Education Institutions [CXQT20013]"

基金资助正文："This work was funded by the National Natural Science Foundation of China (81572080 and 81873972), the Natural Science Foundation Project o f C h o n g q i n g (cstc2015shmszx120086), the Training Program for Advanced Young Medical Personnel of Chongqing (2017HBRC003), the Chongqing Science Fund for Distinguished Young Scholars (cstc2019jcyjjqX0028), and the Foundation for Innovative Research Groups of Chongqing Higher Education Institutions (CXQT20013)."