Generation of a TPM1 homozygous knockout embryonic stem cell line by CRISPR/Cas9 editing

作者全名："Ma, Shuhong; Xu, Qi; Bai, Rui; Dong, Tao; Peng, Zhiping; Liu, Xujie"

作者地址："[Ma, Shuhong; Bai, Rui; Dong, Tao] Capital Med Univ, Anzhen Hosp, Beijing 100029, Peoples R China; [Ma, Shuhong; Bai, Rui; Dong, Tao] Beijing Inst Heart Lung & Blood Vessel Dis, Beijing 100029, Peoples R China; [Xu, Qi] Chinese Acad Med Sci & Peking Union Med Coll, Fuwai Hosp, Natl Ctr Cardiovasc Dis, State Key Lab Cardiovasc Dis, Beijing 100037, Peoples R China; [Peng, Zhiping; Liu, Xujie] Chongqing Med Univ, Basic Med Sch, Dept Radiol, Chongqing 400016, Peoples R China"

通信作者："Liu, XJ (corresponding author), Chongqing Med Univ, Basic Med Sch, Dept Radiol, Chongqing 400016, Peoples R China."

来源：STEM CELL RESEARCH

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000701768700004

JCR分区：Q4

影响因子：1.2

年份：2021

卷号：55

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Alpha-Tropomyosin (TPM1) plays a crucial role in actin regulation and stability and contributes fundamental functions to heart development: without TPM1 expressing, mice embryos will die early in embryogenesis. To further identify the role of TPM1 in human cardiac development, here we generated a homozygous TPM1 knockout (TPM1(-/-)) human embryonic stem cell (hESC) line using CRISPR/Cas9-based genome editing system. The generated TPM1(-/-) hESC line maintained normal karyotype, highly expressed pluripotency markers and was able to differentiate into all three germ layers in vivo. This cell line provides a powerful tool to investigate the role of TPM1 in heart development in future."

基金机构：Beijing Natural Science FoundationBeijing Natural Science Foundation [Z190013]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81970205]; Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences [2019PT320026]

基金资助正文："This work was financially supported by Beijing Natural Science Foundation No. Z190013, National Natural Science Foundation of China Nos. 81970205, and Non-profit Central Research Institute Fund of Chi-nese Academy of Medical Sciences (2019PT320026)."