Effect Of XBP1 Deficiency In Cartilage On The Regulatory Network Of LncRNA/circRNA-miRNA-mRNA

作者全名："Li, Xiaoli; Yang, Yuyou; Liang, Li; Fan, Mengtian; Li, Xingyue; Feng, Naibo; Pan, Yiming; Tan, Qiaoyan; Xu, Qingbo; Xie, Yangli; Guo, Fengjin"

作者地址："[Li, Xiaoli; Yang, Yuyou; Liang, Li; Fan, Mengtian; Li, Xingyue; Feng, Naibo; Pan, Yiming; Guo, Fengjin] Chongqing Med Univ, Dept Cell Biol & Genet, Core Facil Dev Biol, Chongqing 400016, Peoples R China; [Tan, Qiaoyan; Xie, Yangli] Army Med Univ, State Key Lab Trauma Burns & Combined, Dept Wound Repair & Rehabil Med, Trauma Ctr,Daping Hosp,Res Inst Surg, Chongqing 400042, Peoples R China; [Xu, Qingbo] Kings Coll London BHF Ctr, Cardiovasc Div, London, England"

通信作者："Guo, FJ (通讯作者)，Chongqing Med Univ, Dept Cell Biol & Genet, Core Facil Dev Biol, Chongqing 400016, Peoples R China."

来源：INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000741522700009

JCR分区：Q1

影响因子：9.2

年份：2022

卷号：18

期号：1

开始页：315

结束页：330

文献类型：Article

关键词：XBP1; whole transcriptome analysis; cartilage; circRNA-miRNA-mRNA; lncRNA-miRNA-mRNA

摘要："X-box binding protein 1(XBP1) is a critical component for unfolded protein response (UPR) in ER stress. According to previous studies performed with different XBP1-deficient mice, the XBP1 gene affects mouse cartilage development and causes other related diseases. However, how the complete transcriptome, including mRNA and ncRNAs, affects the function of cartilage and other tissues when XBP1 is deficient in chondrocytes is unclear. In this study, we aimed to screen the differentially expressed (DE) mRNAs, circRNAs, lncRNAs and miRNAs in XBP1 cartilage-specific knockout (CKO) mice using high throughput sequencing and construct the circRNA-miRNA-mRNA and lncRNA-miRNA-mRNA and mRNAs [differentially expressed genes (DEGs)] between the cartilage tissue of XBP1 CKO mice and controls were identified, including 441 DE-LncRNAs, 15 DE-circRNAs, 6 DE-miRNAs, and 477 DEGs. Further, 253,235 lncRNA-miRNA-mRNA networks and 1,822 circRNA-miRNA-mRNA networks were constructed based on the correlation between lncRNAs/circRNAs, miRNAs, mRNAs. The whole transcriptome analysis revealed that XBP1 deficiency in cartilage affects the function of cartilage and other different tissues, as well as associated diseases. Overall, our findings may provide potential biomarkers and mechanisms for the diagnosis and treatment of cartilage and other related diseases."

基金机构："National Natural Science Foundation of China [81672209, 81871769]; Chongqing Science and Technology Bureau [cstc2020jcyj-msxmX0175]; Chongqing Human Resources and Social Security Bureau [2018-389]"

基金资助正文："We thank Bingxiang Xu (Shanghai University of Sport) for his help and advice in the data analysis work. This work was aided by the National Natural Science Foundation of China (No. 81672209, No. 81871769); Chongqing Science and Technology Bureau (cstc2020jcyj-msxmX0175); Chongqing Human Resources and Social Security Bureau (2018-389)."