Association between serum miR-221-3p and intravenous immunoglobulin resistance in children with Kawasaki disease

作者全名："Jing, Fengchuan; Weng, Haobo; Pei, Qiongfei; Zhang, Jing; Liu, Ruixi; Yi, Qijian"

作者地址："[Jing, Fengchuan; Weng, Haobo; Pei, Qiongfei; Zhang, Jing; Liu, Ruixi; Yi, Qijian] Chongqing Med Univ, Dept Cardiovasc Med, Childrens Hosp, Chongqing 400014, Peoples R China; [Jing, Fengchuan; Weng, Haobo; Pei, Qiongfei; Zhang, Jing; Liu, Ruixi; Yi, Qijian] Chongqing Med Univ, Minist Educ Key Lab Child Dev & Disorders, Childrens Hosp, Chongqing 400014, Peoples R China; [Jing, Fengchuan; Weng, Haobo; Pei, Qiongfei; Zhang, Jing; Liu, Ruixi; Yi, Qijian] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Childrens Hosp, Chongqing 400014, Peoples R China; [Jing, Fengchuan; Weng, Haobo; Pei, Qiongfei; Zhang, Jing; Liu, Ruixi; Yi, Qijian] Chongqing Med Univ, China Int Sci & Technol Cooperat Base Child Dev &, Childrens Hosp, Chongqing 400014, Peoples R China; [Liu, Ruixi; Yi, Qijian] Chongqing Med Univ, Chongqing Key Lab Pediat, Childrens Hosp, Chongqing 400014, Peoples R China"

通信作者："Liu, RX; Yi, QJ (通讯作者)，Chongqing Med Univ, Dept Cardiovasc Med, Childrens Hosp, Chongqing 400014, Peoples R China.; Liu, RX; Yi, QJ (通讯作者)，Chongqing Med Univ, Minist Educ Key Lab Child Dev & Disorders, Childrens Hosp, Chongqing 400014, Peoples R China.; Liu, RX; Yi, QJ (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Childrens Hosp, Chongqing 400014, Peoples R China.; Liu, RX; Yi, QJ (通讯作者)，Chongqing Med Univ, China Int Sci & Technol Cooperat Base Child Dev &, Childrens Hosp, Chongqing 400014, Peoples R China.; Liu, RX; Yi, QJ (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Pediat, Childrens Hosp, Chongqing 400014, Peoples R China."

来源：CLINICAL AND EXPERIMENTAL MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000744411600001

JCR分区：Q2

影响因子：4.6

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Kawasaki disease; microRNA-221; Intravenous immunoglobulin resistance

摘要："Objectives Intravenous immunoglobulin (IVIG) resistance was a major cause of coronary artery lesions in children with Kawasaki disease (KD). However, the cause of IVIG resistance in KD remains unknown. miR-221-3p has been confirmed involved in cardiovascular diseases and rheumatoid arthritis. The purpose of this study was to investigate the association between miR-221-3p and IVIG resistance in children with KD. Methods Fifty-five KD patients and 29 healthy controls (HCs) were enrolled in this study. KD patients were divided into group of sensitive to IVIG (IVIG-response, n = 42) and group of resistant to IVIG (IVIG-resistance, n = 13), group of 10 KD patients with coronary artery lesions (CALs, KD-CALs) and group of 10 sex- and age-matched KD patients without CALs (KD-NCALs). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the levels of miR-221-3p. Results Compared with the HCs group, miR-221-3p were significantly increased in the KD group (p < 0.05), and the IVIG-resistance group had higher levels of miR-221-3p than those in the IVIG-response group (p < 0.05). CRP (C-reactive protein), PCT (procalcitonin), NLR (neutrophil-lymphocyte ratio) were positively correlated with miR-221-3p in KD patients. In addition, the group of IVIG resistance had a higher level of Kobayashi Score (p < 0.001). The receiver operating characteristic curve showed that miR-221-3p had a better value for diagnosis IVIG resistance in children with KD than Kobayashi Score with the AUC of 0.811 (95% CI, 0.672-0.951), 0.793 (95% CI, 0.618-0.968), respectively. Additionally, miR-221-3p was elevated (p < 0.05) and showed an AUC value of 0.83 (95% CI, 0.648-1.000, p < 0.05) for the prediction of the complication of coronary artery abnormalities in the group of KD with CALs. Conclusions miR-221-3p might be involved in the pathogenesis of KD and IVIG resistance and miR-221-3p can be used as a new potential biomarker to predict IVIG resistance in children with KD."

基金机构：Outstanding Young Talent Training Program Class B of Children's Hospital Affiliated to Chongqing Medical University

基金资助正文：This work was supported by the Outstanding Young Talent Training Program Class B of Children's Hospital Affiliated to Chongqing Medical University; Award Number: None; Recipient: DR. Ruixi Liu.