Study on plasma exosome biomarkers of pregnant women with intrahepatic cholestasis of pregnancy

作者全名："Feng, Fan; Jiang, Mei; Liu, Wei; Xu, Di; Huang, Xiao-Mei; Shao, Yong"

作者地址："[Feng, Fan; Liu, Wei; Xu, Di; Huang, Xiao-Mei; Shao, Yong] Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Chongqing, Peoples R China; [Jiang, Mei] First Peoples Hosp Neijiang, Dept Obstet & Gynecol, Neijiang, Peoples R China"

通信作者："Shao, Y (通讯作者)，Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Chongqing, Peoples R China."

来源：SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000750082500001

JCR分区：Q3

影响因子：1.9

年份：2022

卷号：57

期号：6

开始页：696

结束页：704

文献类型：Article

关键词：Intrahepatic cholestasis of pregnancy; exosome; cluster protein; bioinformatics analysis

摘要："Objective and aims Serum total bile acid (TBA) level as the main index for the diagnosis of intrahepatic cholestasis of pregnancy (ICP) has some limitations. The early diagnosis and new treatment of ICP still need to be further strengthened. Materials and methods Plasma samples were collected, and exosomes were isolated. Key differential proteins were screened by bioinformatics methods. ELISA method was used to detect the concentration of the key differential protein in plasma samples, and the receiver operating characteristic curve (ROC) curve was drawn to find out the best critical value. Results There were 138 differentially expressed proteins between the ICP and the normal groups by quantitative analysis. Cluster protein (CLU) was screened as a clinical validation index. The CLU concentration of plasma exosomes in the ICP group was significantly higher than that in the normal group (p < .0001). ROC curve analysis showed that the best critical point for diagnosing ICP according to the plasma exosomes CLU concentration of pregnant women was 255.28 ng/ml. In the ICP group, the best crucial point for predicting ICP with premature delivery is 286.72 ng/ml. Conclusions The plasma exosomes CLU in pregnant women with ICP is an important biomarker for clinical diagnosis and prediction of premature delivery of ICP."

基金机构：National Natural Sciences Foundation of China [81471473]; Chongqing Health Planning Commission project [2019ZDXM055]

基金资助正文：This work is funded by the National Natural Sciences Foundation of China (No. 81471473) and the Chongqing Health Planning Commission project (No. 2019ZDXM055).