Regulation of ECP fimbriae-related genes by the transcriptional regulator RcsAB in Klebsiella pneumoniae NTUH-K2044

作者全名："Shen, Lifei; Zhang, Jiaxue; Xue, Jian; Du, Ling; Yuan, Lingyue; Nie, Hao; Dai, Sue; Yu, Qian; Li, Yingli"

作者地址："[Shen, Lifei; Du, Ling; Yuan, Lingyue; Nie, Hao; Dai, Sue; Yu, Qian; Li, Yingli] Chongqing Med Univ, Sch Publ Hlth & Management, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China; [Zhang, Jiaxue] Chongqing Jiangbei Dist Ctr Dis Control & Prevent, Chongqing, Peoples R China; [Xue, Jian] Zunyi Med & Pharmaceut Coll, Zunyi, Guizhou, Peoples R China"

通信作者："Li, YL (通讯作者)，Chongqing Med Univ, Sch Publ Hlth & Management, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：JOURNAL OF BASIC MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000752205200001

JCR分区：Q3

影响因子：3.1

年份：2022

卷号：62

期号：5

开始页：593

结束页：603

文献类型：Article

关键词：ECP fimbriae-related gene; Klebsiella pneumoniae; RcsAB; transcriptional regulator; ykgK gene

摘要："Klebsiella pneumoniae is one of the major pathogens causing nosocomial infections. The regulator of capsule synthesis (Rcs) system is a complex signal transduction pathway that is involved in the regulation of virulence factors of K. pneumoniae as an important transcriptional regulator. The RcsAB box-like sequence was found to be present in the promoter-proximal regions of ykgK, one of the ECP fimbriae-related genes, which suggested the expression of ECP fimbriae may be regulated by RcsAB. The ykgK gene in K. pneumoniae has 86% similarity to the ecpR gene in Escherichia coli. Nucleotide sequence alignment revealed a similar ECP fimbriae gene cluster including six genes in K. pneumoniae, which was proved to be on the same operon in this study. The electrophoretic mobility shift assay and DNase I assay, relative fluorescence expression, beta-galactosidase activity, and relative gene expression of ykgK in the wild-type and mutant strains were performed to determine the transcriptional regulation mechanism of RcsAB on ECP fimbriae. The mutant Delta ykgK and complementary strain Delta ykgK/c Delta ykgK were constructed to complete the Galleria mellonella larvae infection experiment and biofilm formation assay. This study showed that RcsAB binds directly to the promoter region of the ykgK gene to positively regulate ECP fimbriae-related gene clusters, and then positively affect the biofilm formation."

基金机构：Natural Science Foundation of Chongqing Science and Technology Department [cstc2020jcyj-msxmX0540]; United funds of unyi Science and Technology Department; Zunyi Medical and Pharmaceutical College [(2018)52]

基金资助正文：This study was supported by the Natural Science Foundation of Chongqing Science and Technology Department (Grant no. cstc2020jcyj-msxmX0540); United funds of unyi Science and Technology Department and Zunyi Medical and Pharmaceutical College (Grant no. (2018)52).