CircLphn3 protects the blood-brain barrier in traumatic brain injury

作者全名："Cheng, Yu-Qi; Wu, Chen-Rui; Du, Meng-Ran; Zhou, Qiang; Wu, Bi-Ying; Fu, Jia-Yuan-Yuan; Balawi, Ehab; Tan, Wei-Lin; Liao, Zheng-Bu"

作者地址："[Cheng, Yu-Qi; Wu, Chen-Rui; Du, Meng-Ran; Zhou, Qiang; Wu, Bi-Ying; Fu, Jia-Yuan-Yuan; Balawi, Ehab; Tan, Wei-Lin; Liao, Zheng-Bu] Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 1, Chongqing, Peoples R China"

通信作者："Liao, ZB (通讯作者)，Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 1, Chongqing, Peoples R China."

来源：NEURAL REGENERATION RESEARCH

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000754512500027

JCR分区：Q2

影响因子：6.1

年份：2022

卷号：17

期号：4

开始页：812

结束页：+

文献类型：Article

关键词：bioinformatics; blood-brain barrier; circRNA; miRNA; RNA-sequence; tight junction protein; traumatic brain injury; ZO1

摘要："Circular RNAs (circRNAs) are a new and large group of non-coding RNA molecules that are abundantly expressed in the central nervous system. However, very little is known about their roles in traumatic brain injury. In this study, we firstly screened differentially expressed circRNAs in normal and injured brain tissues of mice after traumatic brain injury. We found that the expression of circLphn3 was substantially decreased in mouse models of traumatic brain injury and in hemin-treated bEnd.3 (mouse brain cell line) cells. After overexpressing circLphn3 in bEnd.3 cells, the expression of the tight junction proteins, ZO-1, ZO-2, and occludin, was upregulated, and the expression of miR-185-Sp was decreased. In bEnd.3 cells transfected with miR-185-Sp mimics, the expression of ZO-1 was decreased. Dual-luciferase reporter assays showed that circLphn3 bound to miR-185-5p, and that miR-185-5p bound to ZO-1. Additionally, circLphn3 overexpression attenuated the hemin-induced high permeability of the in vitro bEnd.3 cell model of the blood-brain barrier, while miR-185-5p transfection increased the permeability. These findings suggest that circLphn3, as a molecular sponge of miR-185-5p, regulates tight junction proteins' expression after traumatic brain injury, and it thereby improves the permeability of the blood-brain barrier."

基金机构：National Natural Science Foundation of China [81771355]; Natural Science Foundation of Chongqing of China [CSTC2015jcyjA10096]

基金资助正文："This study was supported by the National Natural Science Foundation of China, No. 81771355; and the Natural Science Foundation of Chongqing of China, No. CSTC2015jcyjA10096 (both to ZBL). The funding sources had no role in study conception and design, data analysis or interpretation, paper writing or deciding to submit this paper for publication."