Establishment of an HPLC Method for Determination of Coumarin-3-Carboxylic Acid Analogues in Rat Plasma and a Preliminary Study on Their Pharmacokinetics

作者全名："Xiong, Yan; Liu, Yong-Hong; Li, Jian-Sha; Zhang, Yu-Ying; Zhang, Jing; Gong, Tao; Jiang, Xin-Hui"

作者地址："[Xiong, Yan; Liu, Yong-Hong; Li, Jian-Sha; Zhang, Yu-Ying; Zhang, Jing; Gong, Tao; Jiang, Xin-Hui] Chongqing Med Univ, Chongqing Res Ctr Pharmaceut Engn, Sch Pharm, Chongqing 400016, Peoples R China"

通信作者："Jiang, XH (通讯作者)，Chongqing Med Univ, Chongqing Res Ctr Pharmaceut Engn, Sch Pharm, Chongqing 400016, Peoples R China."

来源：JOURNAL OF CHROMATOGRAPHIC SCIENCE

ESI学科分类：CHEMISTRY

WOS号：WOS:000756424700001

JCR分区：Q4

影响因子：1.3

年份：2022

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期号：　

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文献类型：Article; Early Access

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摘要："A simple high performance liquid chromatography (HPLC) method was developed and validated for the determination of coumarin-3-carboxylic acid analogues (C3AA) in rat plasma and a preliminary study on pharmacokinetics. Ferulic acid (FA) was used as the internal standard substance, and coumarin-3-carboxylic acid (C3A) was used as a substitute for quantitative C3AA. After protein precipitation with methanol, the satisfactory separation was achieved on an ODS2 column when the temperature was maintained at 30 +/- 2 degrees C. The correlation coefficient r in the C3A linear equation is equal to 0.9990. Pharmacokinetic parameters for t(1/2), T-max, C-max, area under the curve (AUC)(0-t), average residence time (MRT), apparent volume of distribution (V z/F) and clearance (Cl/F) were 1.89 +/- 0.03 h, 0.39 +/- 0.14 h, 1.81 +/- 0.10 g center dot mL(-1) center dot h, 7.88 +/- 0.24 g center dot mL(-1)center dot h, 3.23 +/- 0.14 h, 0.43 +/- 0.03 (mg center dot kg(-1))center dot(g center dot mL(-1))(-1)center dot h(-1), respectively. The high performance liquid chromatography-photo diode array detector (HPLC-PDA) method established in this study can be used to separate and determine the content of C3AA in plasma of rats after 60% ethanol extraction by gavage. The plasma concentration-time curve and pharmacokinetic parameters reflect the absorption of C3AA in rat blood after oral administration to some extent."

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