microRNA-455-5p alleviates neuroinflammation in cerebral ischemia/reperfusion injury

作者全名："Zhang, Jian-Song; Hou, Pin-Pin; Shao, Shuai; Manaenko, Anatol; Xiao, Zhi-Peng; Chen, Yan; Zhao, Bing; Jia, Feng; Zhang, Xiao-Hua; Mei, Qi-Yong; Hu, Qin"

作者地址："[Zhang, Jian-Song; Hou, Pin-Pin; Hu, Qin] Shanghai Jiao Tong Univ, Renji Hosp, Cent Lab, Sch Med, Shanghai, Peoples R China; [Zhang, Jian-Song; Shao, Shuai; Xiao, Zhi-Peng; Zhao, Bing; Jia, Feng; Zhang, Xiao-Hua; Hu, Qin] Shanghai Jiao Tong Univ, Renji Hosp, Dept Neurosurg, Sch Med, Shanghai, Peoples R China; [Manaenko, Anatol] Chongqing Med Univ, Dept Neurol, Affiliated Hosp 1, Chongqing, Peoples R China; [Chen, Yan] Shanghai Jiao Tong Univ, Dept Med Genet, Sch Med, Shanghai, Peoples R China; [Mei, Qi-Yong] Second Mil Med Univ, Changzheng Hosp, Dept Neurosurg, Shanghai, Peoples R China"

通信作者："Hu, Q (通讯作者)，Shanghai Jiao Tong Univ, Renji Hosp, Cent Lab, Sch Med, Shanghai, Peoples R China.; Hu, Q (通讯作者)，Shanghai Jiao Tong Univ, Renji Hosp, Dept Neurosurg, Sch Med, Shanghai, Peoples R China.; Mei, QY (通讯作者)，Second Mil Med Univ, Changzheng Hosp, Dept Neurosurg, Shanghai, Peoples R China."

来源：NEURAL REGENERATION RESEARCH

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000766445300032

JCR分区：Q2

影响因子：6.1

年份：2022

卷号：17

期号：8

开始页：1769

结束页：1775

文献类型：Article

关键词：agomiR-455-5p; biomarker; blood-brain barrier; C-C chemokine receptor type 5; ischemia/reperfusion injury; ischemic stroke; microglia; microRNA-455-5p; neuroinflammation; pretreatment

摘要："Neuroinflammation is a major pathophysiological factor that results in the development of brain injury after cerebral ischemia/reperfusion. Downregulation of microRNA (miR)-455-5p after ischemic stroke has been considered a potential biomarker and therapeutic target for neuronal injury after ischemia. However, the role of miR-455-5p in the post-ischemia/reperfusion inflammatory response and the underlying mechanism have not been evaluated. In this study, mouse models of cerebral ischemia/reperfusion injury were established by transient occlusion of the middle cerebral artery for 1 hour followed by reperfusion. Agomir-455-5p, antagomir-455-5p, and their negative controls were injected intracerebroventricularly 2 hours before or 0 and 1 hour after middle cerebral artery occlusion (MCAO). The results showed that cerebral ischemia/reperfusion decreased miR-455-5p expression in the brain tissue and the peripheral blood. Agomir-455-5p pretreatment increased miR-455-5p expression in the brain tissue, reduced the cerebral infarct volume, and improved neurological function. Furthermore, primary cultured microglia were exposed to oxygen-glucose deprivation for 3 hours followed by 21 hours of reoxygenation to mimic cerebral ischemia/reperfusion. miR-455-5p reduced C-C chemokine receptor type 5 mRNA and protein levels, inhibited microglia activation, and reduced the production of the inflammatory factors tumor necrosis factor-a and interleukin-1 beta. These results suggest that miR-455-5p is a potential biomarker and therapeutic target for the treatment of cerebral ischemia/reperfusion injury and that it alleviates cerebral ischemia/reperfusion injury by inhibiting C-C chemokine receptor type 5 expression and reducing the neuroinflammatory response."

基金机构："National Natural Science Foundation of China [82071283, 81671130]; Medical Engineering Cross Research Foundation of Shanghai Jiao Tong University of China [YG2017MS83]; Shanghai Municipal Science and Technology Commission Medical Guidance Science and Technology Support Project of China [19411968400]"

基金资助正文："This study was supported by the National Natural Science Foundation of China, Nos. 82071283 (to QH) and 81671130 (to QH); Medical Engineering Cross Research Foundation of Shanghai Jiao Tong University of China, No. YG2017MS83 (to QH); and from Shanghai Municipal Science and Technology Commission Medical Guidance Science and Technology Support Project of China, No. 19411968400 (to QYM)."