A Fibrin Site-Specific Nanoprobe for Imaging Fibrin-Rich Thrombi and Preventing Thrombus Formation in Venous Vessels

作者全名："Zhong, Yixin; Ye, Man; Huang, Liandi; Hu, Liu; Li, Fangzhou; Ni, Qiankun; Zhong, Jie; Wu, Hongyun; Xu, Fengfei; Xu, Jie; He, Xiaojing; Wang, Zhigang; Ran, Haitao; Wu, Yunzhu; Guo, Dajing; Liang, Xing-Jie"

作者地址："[Zhong, Yixin; Hu, Liu; Wu, Hongyun; Xu, Jie; He, Xiaojing; Wu, Yunzhu; Guo, Dajing] Chongqing Med Univ, Affiliated Hosp 2, Dept Radiol, 74 Linjiang Rd, Chongqing 400010, Peoples R China; [Zhong, Yixin; Li, Fangzhou; Ni, Qiankun; Zhong, Jie; Xu, Fengfei; Liang, Xing-Jie] Chinese Acad Sci, Key Lab Biomed Effects Nanomat & Nanosafety, Ctr Excellence Nanosci, Natl Ctr Nanosci & Technol China, 11 First North Rd, Beijing 100190, Peoples R China; [Zhong, Yixin; Li, Fangzhou; Ni, Qiankun; Zhong, Jie; Xu, Fengfei; Liang, Xing-Jie] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zhong, Yixin; Huang, Liandi; Wang, Zhigang; Ran, Haitao] Chongqing Med Univ, State Key Lab Ultrasound Med & Engn, Chongqing Key Lab Ultrasound Mol Imaging, Affiliated Hosp 2, 74 Linjiang Rd, Chongqing 400010, Peoples R China; [Zhong, Yixin; Huang, Liandi; Wang, Zhigang; Ran, Haitao] Chongqing Med Univ, Ultrasound Dept, Affiliated Hosp 2, 74 Linjiang Rd, Chongqing 400010, Peoples R China; [Ye, Man] Wuhan Univ, Renmin Hosp, Dept Radiol, 238 Jiefang Rd, Wuhan 430060, Hubei, Peoples R China"

通信作者："Guo, DJ (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Radiol, 74 Linjiang Rd, Chongqing 400010, Peoples R China.; Liang, XJ (通讯作者)，Chinese Acad Sci, Key Lab Biomed Effects Nanomat & Nanosafety, Ctr Excellence Nanosci, Natl Ctr Nanosci & Technol China, 11 First North Rd, Beijing 100190, Peoples R China.; Liang, XJ (通讯作者)，Univ Chinese Acad Sci, Beijing 100049, Peoples R China."

来源：ADVANCED MATERIALS

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000768166300001

JCR分区：Q1

影响因子：29.4

年份：2022

卷号：34

期号：16

开始页：　

结束页：　

文献类型：Article

关键词：fibrin; image-guided therapy; multimodal imaging; targeted nanoprobes; thrombus prevention

摘要："Venous thromboembolism (VTE) is a prevalent public health issue worldwide. Before treatment, spatiotemporally accurate thrombus detection is essential. However, with the currently available imaging technologies, this is challenging. Herein, the development of a novel fibrin-specific nanoprobe (NP) based on the conjugation of poly(lactic-co-glycolic acid) with the pentapeptide Cys-Arg-Glu-Lys-Ala (CREKA) for selective and semiquantitative imaging in vivo is presented. By integrating Fe3O4 and NIR fluorochrome (IR780), the NP can function as a highly sensitive sensor for the direct analysis of thrombi in vivo. The fibrin-specific NP distinguishes fibrin-rich thrombi from collagen-rich or erythrocyte-rich thrombi, which can be beneficial for future individually tailored therapeutic strategy. Furthermore, loading NPs with the ketotifen fumarate results in mast cell degranulation inhibition, and hence, NPs can prevent thrombosis without the risk of excessive bleeding. Thus, the use of fibrin-specific NPs may serve as a safe alternative approach for the detection and prevention of VTEs in susceptible populations in the future."

基金机构："National Key Research & Development Program of China [2021YFA1201000, 2018YFE0117800]; National Natural Science Foundation of China (NSFC) key project [32030060, 81971608]; NSFC international collaboration key project [51861135103]; China Postdoctoral Science Foundation [2019M663891XB, 2020T130761]; Chongqing Postdoctoral Program for Innovative Talents; Chongqing Natural Science Foundation [cstc2021jcyj-bsh0003]; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University [CQYC2020030389, 2020-7]; Construction Program of Chongqing University Innovation Team [CXTDG201602007]; Beijing-Tianjin-Hebei Basic Research Cooperation Project [19JCZDJC64100]; SIEMENS Healthineers Ltd."

基金资助正文："Y.Z. and M.Y. contributed equally to this work. This work was supported by the National Key Research & Development Program of China (grant Nos. 2021YFA1201000 and 2018YFE0117800), National Natural Science Foundation of China (NSFC) key project (grant No. 32030060) and project (grant No. 81971608), NSFC international collaboration key project (grant No. 51861135103). The authors also appreciate the China Postdoctoral Science Foundation (grant Nos. 2019M663891XB, 2020T130761), the Chongqing Postdoctoral Program for Innovative Talents, the Chongqing Natural Science Foundation for postdoctoral (grant No. cstc2021jcyj-bsh0003), the Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University (grant No. CQYC2020030389, 2020-7) and Construction Program of Chongqing University Innovation Team (grant No. CXTDG201602007), and the support by ""the Beijing-Tianjin-Hebei Basic Research Cooperation Project"" (grant No. 19JCZDJC64100). The authors also acknowledge support from the SIEMENS Healthineers Ltd."