Protein-mediated DNA self-assembly by controlling the surface charge in a molecular crowding environment

作者全名："Chen, Chunfa; Liu, Qian; Yuan, Yue; Cai, Xiaolian; Ding, Xiaotong; Li, Boxuan; Yang, Yao; Wang, Bin; Wang, Guansong; Leong, David Tai; Qian, Hang"

作者地址："[Chen, Chunfa; Liu, Qian; Yuan, Yue; Ding, Xiaotong; Yang, Yao; Wang, Bin; Wang, Guansong; Qian, Hang] Third Mil Med Univ, Xinqiao Hosp, Inst Resp Dis, Chongqing 400037, Peoples R China; [Liu, Qian] Chongqing Med Univ, Lab Teaching & Management Ctr, Lab Pharm & Chem, Chongqing 400016, Peoples R China; [Liu, Qian] Chongqing Med Univ, Lab Teaching & Management Ctr, Lab Tissue & Cell Biol, Chongqing 400016, Peoples R China; [Cai, Xiaolian] Third Mil Med Univ, Southwest Hosp, Dept Cardiol, Chongqing 400038, Peoples R China; [Li, Boxuan] Changzhi Med Coll, Dept Pharm, Changzhi 046000, Shanxi, Peoples R China; [Leong, David Tai] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore, Singapore"

通信作者："Wang, GS; Qian, H (通讯作者)，Third Mil Med Univ, Xinqiao Hosp, Inst Resp Dis, Chongqing 400037, Peoples R China.; Leong, DT (通讯作者)，Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore, Singapore."

来源：BIOMATERIALS SCIENCE

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000768938800001

JCR分区：Q2

影响因子：6.6

年份：2022

卷号：10

期号：8

开始页：2006

结束页：2013

文献类型：Article

关键词：　

摘要："Designing and building artificial nanodevices and nanoarchitectures in living systems are extremely intriguing subjects in nanotechnology and synthetic biology. Taking advantage of cellular machinery and endogenous biomacromolecules, such as proteins, is key to achieving the precise and sophisticated manipulation of nanoarchitectures. In this study, we proposed a protein-mediated DNA self-assembly strategy in a molecular crowding environment. By carefully controlling the surface charge of basic nuclear proteins in a crowding environment that mimicked the intracellular environment, we demonstrated that highly positively charged protamine can assemble individual DNA strands into defined structures similar to a catalytic process manner. Successful self-assembly required an optimized protamine surface charge and a crowding environment; otherwise, this self-assembly was impossible. Polyacrylamide gel electrophoresis (PAGE), atomic force microscopy (AFM), and dynamic light scattering (DLS) results showed that tile-based DNA tubular structures, tetrahedra, and two dimensional DNA origami structures were successfully assembled. We inferred that the assembly process occurred between the arginine-rich domain of protamine and DNA strands that repeatedly interacted with each other in the viscous system. The current study provides a potential strategy to construct nanodevices in living systems and presents an alternative protein-DNA interaction for the potential fabrication of protein-DNA hybrid nanomaterials."

基金机构："National Natural Science Foundation of China [32071379, 81670047, 81873422]; Natural Science Foundation of Chongqing, China [cstc2021jcyj-msxmX1179]; Project Foundation of Chongqing Municipal Education Committee [KJQN201900405]"

基金资助正文："We would like to thank Professor Hua Zuo at the College of Pharmaceutical Sciences, Southwest University for her technical support with AFM imaging (Bruker Multimode 8). This work was supported by the National Natural Science Foundation of China (32071379, 81670047, and 81873422), the Natural Science Foundation of Chongqing, China (no. cstc2021jcyj-msxmX1179), and the Project Foundation of Chongqing Municipal Education Committee (KJQN201900405)."