Exosomal microRNA-4535 of Melanoma Stem Cells Promotes Metastasis by Inhibiting Autophagy Pathway

作者全名："Liu, Doudou; Li, Xiaoshuang; Zeng, Bin; Zhao, Qiting; Chen, Hao; Zhang, Yuhan; Chen, Yuting; Wang, Jianyu; Xing, H. Rosie"

作者地址："[Liu, Doudou; Li, Xiaoshuang; Zeng, Bin; Zhao, Qiting; Chen, Hao; Zhang, Yuhan; Chen, Yuting] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China; [Liu, Doudou; Li, Xiaoshuang; Zeng, Bin; Zhao, Qiting; Chen, Hao; Zhang, Yuhan; Chen, Yuting] Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China; [Wang, Jianyu; Xing, H. Rosie] Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China"

通信作者："Wang, JY; Xing, HR (通讯作者)，Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China."

来源：STEM CELL REVIEWS AND REPORTS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000769841100001

JCR分区：Q1

影响因子：4.8

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：cancer stem cells; microRNA 4535; Exosome; cancer metastasis; Metastatic colonization; Autophagy

摘要："High mortality rate and poor survival in melanoma are associated with efficient metastatic colonization. The underlying mechanisms remain elusive. Elucidating the role of exosomes in mediating the interactions between cancer cells and the metastatic microenvironment has been focused on cancer cell derived exosomes in modulating the functions of stromal cells. Whether cancer stem cells (CSCs) can modify the metastatic properties of non-CSC cells, and whether exosomal crosstalk plays a role have not been demonstrated prior to this report. In this study, a paired M14 melanoma derivative cell line, i.e., melanoma parental cell (MPC) and its CSC derivative cell line melanoma stem cell (MSC) were employed. We demonstrated that exosomal crosstalk betwen MSCs and non-CSC MPCs is a new mechanism that underlies melanoma metastasis. Low metastatic melanoma cells (MPCs) can acquire the ""metastatic power"" from highly metastatic melanoma CSCs (MSCs). We illustrated an uncharacterized microRNA, miR-4535 in mediating such exosomal crosstalk. MSCs deliver its exosomal miR-4535 to the targeted MPCs. Upon entering MPCs, miR-4535 augments metastatic colonization of MPCs by inactivating the autophagy pathway."

基金机构："National Natural Science Fund [82073277, 82173247]; Science and Technology Project Affiliated to the Education Department of Chongqing [KJQN202100404]; Natural Science Fund of Chongqing [cstc2019jcyj-msxmX0868]; Science and Technology Project of Chongqing Yuzhong District [20200110]"

基金资助正文："This work was supported by the National Natural Science Fund (Grant No. 82073277 and 82173247), the Science and Technology Project Affiliated to the Education Department of Chongqing (Grant No. KJQN202100404), Natural Science Fund of Chongqing (Grant No. cstc2019jcyj-msxmX0868) and Science and Technology Project of Chongqing Yuzhong District (Grant No. 20200110)."