Circular gap forming device and two-dimensional area calculation for in vitro cell migration study

作者全名："Zhang, Nannan; Fang, Shuyu; Bi, Yang"

作者地址："[Zhang, Nannan; Fang, Shuyu; Bi, Yang] Chongqing Med Univ, Stem Cell Biol & Therapy Lab,Chongqing Key Lab Pe, Childrens Hosp,Minist Educ,Key Lab Child Dev & Di, Natl Clin Res Ctr Child Hlth & Disorders, Bldg 7,Room 905,136 Zhongshan Er Rd, Chongqing 400014, Peoples R China"

通信作者："Bi, Y (通讯作者)，Chongqing Med Univ, Stem Cell Biol & Therapy Lab,Chongqing Key Lab Pe, Childrens Hosp,Minist Educ,Key Lab Child Dev & Di, Natl Clin Res Ctr Child Hlth & Disorders, Bldg 7,Room 905,136 Zhongshan Er Rd, Chongqing 400014, Peoples R China."

来源：CELL AND TISSUE BANKING

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000771892500001

JCR分区：Q4

影响因子：1.5

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Cell migration; Wound healing assay; Circular cell wound

摘要："Cell wound healing assay is an important experimental technique for the detection of cell migration in vitro. At present, scratch on monolayer cells using a pipette tip is commonly used. However, it is difficult to guarantee the scratch with same width, and only the migration distance of a certain part is calculated. Therefore, the experimental method needs to be optimized. ATRA was used to treat hepa1-6 mouse hepatoma cells. Circular wound with diameter of 0.2 cm were formed by a circular gap forming device. The whole cell wound region could be captured under the microscope to observe cell migration. There are almost no crawling cells in the wound region. The migration capacity of hepa1-6 cells was evaluated by calculating the healing area. ATRA could significantly inhibit the migration of hepa1-6 cells. Compared with linear wound, the standard deviation of wound healing rate in the circular cell wound method is smaller. The circular cell wound method can ensure the dynamic observation of the same wound region, and calculate the healing area at the two-dimensional level with small error and high repetition rate. It is reliable and easy to operate, can be widely used in laboratory."

基金机构：Natural scientific research program of Research Project of Chongqing Science and Technology Commission [cstc2021jcyj-msxmX0225]

基金资助正文：This work was supported by the Natural scientific research program of Research Project of Chongqing Science and Technology Commission (No. cstc2021jcyj-msxmX0225 to YB).