A stochastic DNA walker electrochemiluminescence biosensor based on quenching effect of Pt@CuS on luminol@Au/Ni-Co nanocages for ultrasensitive detection of I27L gene

作者全名："Gong, Wei; Shu, Jia; Ju, Pan; Wu, Shaoyan; Chen, Yuzhou; Ding, Shijia; Yang, Wei; Luo, Rong"

作者地址："[Gong, Wei; Ju, Pan; Wu, Shaoyan; Luo, Rong] Chongqing Med Univ, Affiliated Hosp 1, Med Examinat Ctr, Chongqing 400016, Peoples R China; [Shu, Jia] Chongqing Med Univ, Affiliated Hosp 1, Dept Ophthalmol, Chongqing 40016, Peoples R China; [Chen, Yuzhou; Yang, Wei] Zhejiang Prov Peoples Hosp, Dept Clin Lab, Hangzhou Med Coll, Peoples Hosp, Hangzhou 310014, Peoples R China; [Ding, Shijia] Chongqing Med Univ, Key Lab Clin Lab Diagnost, Minist Educ, Coll Lab Med, Chongqing 400016, Peoples R China"

通信作者："Luo, R (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Med Examinat Ctr, Chongqing 400016, Peoples R China.; Yang, W (通讯作者)，Zhejiang Prov Peoples Hosp, Dept Clin Lab, Hangzhou Med Coll, Peoples Hosp, Hangzhou 310014, Peoples R China."

来源：CHEMICAL ENGINEERING JOURNAL

ESI学科分类：ENGINEERING

WOS号：WOS:000773397100002

JCR分区：Q1

影响因子：15.1

年份：2022

卷号：434

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Electrochemiluminescence resonance energy; transfer; Luminol; Prussian blue analogs; CuS nanospheres; I27L gene

摘要："The common I27L mutations of the hepatocyte nuclear factor-1 alpha (HNF1A) significantly increase the incidence of maturity-onset diabetes of the young (MODY). Hence, the establishment of an ultrasensitive detection strategy for I27L gene is crucial for the diagnosis and treatment of MODY. Herein, a signal-off electrochemiluminescence resonance energy transfer (ECL-RET) biosensor with effective target-induced stochastic DNA walker amplification was fabricated for I27L detection. In this biosensor, Prussian blue analogues (Ni-Co) nanocages/Ausupported luminol (luminol@Au/Ni-Co) were used as ECL donors and exhibited excellent luminous efficiency. On the other hand, Pt nanoparticles-grown on copper sulphide (Pt@CuS) were used as energy acceptors to quench the initial signal based on RET between luminol and CuS. Moreover, the autonomous locomotion of I27L assisted by Exo III is the key in this DNA walker biosensor, as it continually induces new digestion processes on the electrode surface and quenches signals caused by the introduction of numerous acceptors. The efficient donor/acceptor pairs and DNA walker amplification system of the ECL-RET allowed the proposed biosensor to effectively detect I27L with a wide linear range of 0.0001 to 100 nM and a limit of detection as low as 23 fM. Thus, the ECL-RET biosensor provides a promising sensing platform for the diagnosis and typing of diabetes."

基金机构：National Science and Technology Major Project of the Ministry of Science and Technology of China [2018ZX10732202]; National Natural Science Foundation of China [81873980]

基金资助正文："Acknowledgements This research was funded by the financial support from the National Science and Technology Major Project of the Ministry of Science and Technology of China (2018ZX10732202) , and the National Natural Science Foundation of China (81873980) ."