A new biomarker neuropeptide Y and bioinformatics analysis of intrahepatic cholestasis of pregnancy

作者全名："Feng, Fan; Xu, Di; Shao, Yong"

作者地址："[Feng, Fan; Xu, Di; Shao, Yong] Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Chongqing, Peoples R China"

通信作者："Shao, Y (通讯作者)，Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Chongqing, Peoples R China."

来源：JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000777002200001

JCR分区：Q3

影响因子：1.6

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：bioinformatics analysis; intrahepatic cholestasis of pregnancy; neuropeptide Y

摘要："Aim To explore the expression of neuropeptide Y (NPY) and bioinformatics characteristics of intrahepatic cholestasis of pregnancy (ICP). Methods Gene chip data of intrahepatic cholestasis of pregnancy were searched from the GEO database with bioinformatics method, and GSE46157 gene chip was downloaded. Differentially expressed genes in normal pregnant placenta tissue and ICP pregnant placenta tissue (bile acid concentration > 40 mu mol/L) were screened by GEO2R. Functional annotation (GO) and pathway analysis (KEGG) were performed with DAVID. STRING online database and Cytoscape software were used for protein interaction network analysis. Maternal serum NPY level of 63 cases of ICP pregnant women and 20 normal pregnant women were investigated by ELISA. Results After screening 3896 differential genes and protein interaction, the top 14 hub genes were selected with nine up-regulated and five down-regulated genes. ICP patients were divided into three subgroups according to serum TBA and ALT levels. Maternal serum NPY levels of pregnant women in ICP subgroups 1, 2, and 3 were significantly higher than those in the normal pregnant women. The number of premature births, meconium-staining amniotic fluid, neonatal asphyxia, and NICU admission was significantly higher in the ICP subgroup 1 than in the ICP subgroups 2 and 3, and than in the normal pregnant women. Conclusion This study indicates that many differentially expressed genes and signaling pathways are involved in the pathophysiological procedure of ICP. NPY could be a new biomarker of ICP."

基金机构：National Natural Sciences Foundation of China [81471473]; Chongqing Health Planning Commission project [2019ZDXM055]

基金资助正文："We would like to thank all the patients and their families for participating in this study and allowing this publication. We are grateful for all the funding supporters, including the National Natural Sciences Foundation of China (No: 81471473) and the Chongqing Health Planning Commission project (No. 2019ZDXM055)."