Circular RNA circ_0001445 alleviates the ox-LDL-induced endothelial injury in human primary aortic endothelial cells through regulating ABCG1 via acting as a sponge of miR-208b-5p

作者全名："Yang, Zhihua; Liang, Xing; Yang, Lixia"

作者地址："[Yang, Zhihua; Yang, Lixia] 920 Hosp Joint Logist Support Force, Dept Cardiovasc Med, 212 Daguan Rd, Kunming, Yunnan, Peoples R China; [Liang, Xing] Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 2, 288 Tianwen Ave, Chongqing, Peoples R China"

通信作者："Yang, LX (通讯作者)，920 Hosp Joint Logist Support Force, Dept Cardiovasc Med, 212 Daguan Rd, Kunming, Yunnan, Peoples R China."

来源：GENERAL THORACIC AND CARDIOVASCULAR SURGERY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000779227000002

JCR分区：Q3

影响因子：1.2

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Circ_0001445; Mir-208b-5p; ABCG1; Coronary artery disease; Ox-LDL

摘要："Background Coronary artery disease (CAD) originates from the blockage of the inner walls of the coronary arteries due to a plaque buildup. Circular RNA (circRNA) circ_0001445 has been reported to be downregulated in patients with a higher coronary atherosclerotic burden. This study is designed to explore the role and mechanism of circ_0001445 on the oxidized low-density lipoprotein (ox-LDL)-induced endothelial cell damage. Methods Circ_0001445, microRNA-208b-5p (miR-208b-5p), and ATP-binding cassette sub-family G member 1 (ABCG1) levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Inflammatory cytokines levels, cell viability, proliferation, migration were detected by Enzyme-linked immunosorbent assay (ELISA) kits, Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), and transwell assays, respectively. Protein levels were determined by western blot assay. The binding between miR-208b-5p and circ_0001445 or ABCG1 was predicted by circBank or TargetScan, and then verified by a dual-luciferase reporter, RNA Immunoprecipitation (RIP), and RNA pull-down assays. Results Circ_0001445 and ABCG1 were decreased, and miR-208b-5p was increased in CAD patients and ox-LDL-treated HAECs. Also, circ_0001445 overexpression could weaken ox-LDL-triggered HAEC injury by boosting proliferation, migration, and repressing inflammation and extracellular matrix (ECM). Mechanically, circ_0001445 directly targeted miR-208b-5p. Furthermore, miR-208b-5p mediated the modulation of circ_0001445 in ox-LDL-induced HAEC injury. ABCG1 acted as a direct target of miR-208b-5p, and the downregulation of miR-208b-5p relieved ox-LDL-induced HAEC damage by interacting with ABCG1. Additionally, circ_0001445 regulated ABCG1 expression by sponging miR-208b-5p. Conclusion Circ_0001445 could abate ox-LDL-mediated HAEC damage by the miR-208b-5p/ABCG1 axis, providing a novel insight into the pathogenesis and treatment of CAD."

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